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AM03T Anti-Bak (Ab-1) Mouse mAb (TC-100)

Anti-Bak (Ab-1) Mouse mAb (TC-100) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: bcl-2 Antagonist Killer

AM03T
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~30 (apparent MW) kDa Bak protein in HL-60 cells.
Catalogue NumberAM03T
Brand Family Calbiochem®
Synonymsbcl-2 Antagonist Killer
References
ReferencesChittenden, T., et al. 1995. Nature 374, 733.
Farrow, S.N., et al. 1995. Nature 374, 731.
Kiefer, M.C., et al. 1995. Nature 736.
Reed, J.C. 1994. J. Cell Biol. 124, 1.
Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol, pH 7.5.
Positive controlHL-60 cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (2.5 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems for each application.
Biological Information
Immunogenrecombinant human Bak protein from which the C-terminal and transmembrane domains have been deleted
ImmunogenHuman
Epitopewithin the N-terminal region
CloneTC-100
HostMouse
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AM03T 0

Documentation

Anti-Bak (Ab-1) Mouse mAb (TC-100) Certificates of Analysis

TitleLot Number
AM03T

References

Reference overview
Chittenden, T., et al. 1995. Nature 374, 733.
Farrow, S.N., et al. 1995. Nature 374, 731.
Kiefer, M.C., et al. 1995. Nature 736.
Reed, J.C. 1994. J. Cell Biol. 124, 1.
Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-August-2007 RFH
Synonymsbcl-2 Antagonist Killer
ApplicationImmunoblotting (2.5 µg/ml)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~30 (apparent MW) kDa Bak protein.
BackgroundBak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. Bak was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4 kDa but migrates by gel electrophoresis as an approximately 30 kDa protein under denaturing conditions. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA3. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner, has been found in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant human Bak protein from which the C-terminal and transmembrane domains have been deleted
Epitopewithin the N-terminal region
CloneTC-100
IsotypeIgG2a
Specieshuman, not mouse, not rat
Positive controlHL-60 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems for each application.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChittenden, T., et al. 1995. Nature 374, 733.
Farrow, S.N., et al. 1995. Nature 374, 731.
Kiefer, M.C., et al. 1995. Nature 736.
Reed, J.C. 1994. J. Cell Biol. 124, 1.
Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.