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MAB4155 Anti-BCRP1 Antibody, clone 5D3

MAB4155
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RFC, ICC, IF, FUNC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4155
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-BCRP1 Antibody, clone 5D3
Alternate Names
  • ABC transporter
  • ATP-binding cassette transporter G2
  • ATP-binding cassette, sub-family G (WHITE), member 2
  • ATP-binding cassette, sub-family G, member 2
  • Breast cancer resistance protein
  • CD338 antigen
  • Mitoxantrone resistance-associated protein
  • Placenta-specific ATP-binding cassette transporter
  • mitoxantrone resistance protein
  • placenta specific MDR protein
Background InformationBreast cancer resistance protein (BCRP1), also known as placenta-specific ATP-binding cassette (ABC) protein (ABCP) or ABC G-subfamily member 2 (ABCG2), or mitoxantrone resistance protein (MXR), is a member of the ATP-binding cassette superfamily of drug transporters thought to be involved in multi-drug resistance in human neoplastic disease. ABCG2 is expressed in drug-resistant breast, colon and gastric cancer and fibrosarcoma cancer cell lines and in blast cells from patients with acute myeloid leukemia (AML). Normal tissues that express the ABCG2 protein include placental syncytiothrophoblasts, mammary ducts and lobules, epithelium in the large intestine and colon, venous and capillary epithelium and bile canalicular membrane of the liver. Several studies indicate that ABCG2 is also expressed in stem cells of different tissues and may be useful as a stem cell marker. ABCG2 is thought to be responsible for the ability of a population of very primitive hematopoietic and non-hematopoietic cells, the so-called SP (side population) subset, to efflux the Hoechst 33342 fluorescent dye. Semiquantitative RT-PCR results indicate that ABCG2 mRNA is expressed at higher levels in SP cells as compared to non-SP cells in human, rhesus monkey and mouse hematopoietic tissues, with the exception of erythroblasts and NK cells. In human bone marrow, ABCG2 mRNA expression is higher in phenotypically defined populations of primitive CD34+CD38- hematopoietic cells as compared to more differentiated CD34+CD38+ progenitor cells. Bcrp1 mRNA is also expressed in SP cells in murine skeletal muscle, in neural stem cell and progenitor cells and in nestin-positive pancreatic islet derived progenitor cells. Over-expression of the human ABCG2 gene by retroviral-mediated gene transfer confers the SP phenotype to mouse bone marrow cells. Bcrp1 mRNA is expressed in SP as well as non-SP fractions of mouse embryonic stem cells. This suggests that Bcrp1 protein levels may not always be reflected by mRNA levels, or that other characteristics of stem cells, for example, cell cycle status or expression of other transporters, are also involved in the SP phenotype.
References
Product Information
FormatPurified
Control
  • MCF-7 cells
PresentationPurified mouse monoclonal IgG2bκ liquid in buffer containing PBS, pH 7.4, with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect BCRP1 using this Anti-BCRP1 Antibody, clone 5D3 validated for use in FC, IC, IF, FUNC & WB.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Affects Function
  • Western Blotting
Application NotesImmunocytochemistry:
Cell cultures fixed with 4% PFA 5-15 minutes at room temperature. Blocking with 10% NGS, 0.1% BSA in PBS with 0.3% Triton X-100 (Ginis et al., 2004).

Flow Cytometry:
1:100-1:1000 dilution from a previous lot was used.

Inhibition Assay:
A previous lot was used to inhibit ABCG2 (Watanabe, K. et al., (2004. FEBS Letters 565:6-10). See also Sarkadi, B., et al., 2004 FEBS 567:116-120,. Sarkadi reports that the detection and inhibition by 5D3 strongly depends on the actual conformation of ABCG2.
Blocking with 10% NGS, 0.1% BSA in PBS with 0.3% triton X-100 (Ginis, 2004).

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen3T3 cells expressing human ABCG2
Clone5D3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificitySpecifically recognizes an external epitope of the human form of ABCG2. Reportedly reacts with mouse stem cell lines (Ginis, et al., 2004), however mouse reactivity is unsupported at this time.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
Gene Symbol
  • ABCG2
  • BCRP
  • BMDP
  • BCRP1
  • MXR
  • ABC15
  • ABCP
  • EST157481
  • MGC102821
  • MXR1
  • MRX
  • CDw338
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UNQ0 # Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123.
SIZE: 655 amino acids; 72314 Da
SUBUNIT: Monomer or homodimer; disulfide-linked.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in placenta. Low expression in small intestine, liver and colon.
PTM: Glycosylation-deficient ABCG2 is normally expressed and functional.
SIMILARITY: SwissProt: Q9UNQ0 ## Belongs to the ABC transporter family. ABCG (White) subfamily. & Contains 1 ABC transporter domain.
Stem Cell Type
  • Neural Stem Cells
  • Hematopoietic Stem Cells
Molecular Weight72 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected BCRP1 on 10 μg of L6 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt. Do not freeze.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB4155 04053252266621

Documentation

Anti-BCRP1 Antibody, clone 5D3 SDS

Title

Safety Data Sheet (SDS) 

Anti-BCRP1 Antibody, clone 5D3 Certificates of Analysis

TitleLot Number
Anti-BCRP, clone 5D3 - LV1492897 LV1492897
Anti-BCRP, clone 5D3 - LV1657474 LV1657474
Anti-BCRP, clone 5D3 - LV1764706 LV1764706
Anti-BCRP, clone 5D3 - NG1854092 NG1854092
Anti-BCRP1, clone 5D3 - 2121764 2121764
Anti-BCRP1, clone 5D3 - 2430539 2430539
Anti-BCRP1, clone 5D3 - 2016624 2016624
Anti-BCRP1, clone 5D3 - 2246772 2246772
Anti-BCRP1, clone 5D3 - 2322538 2322538
Anti-BCRP1, clone 5D3 - 3241221 3241221

References

Reference overviewPub Med ID
Comparative proteomics reveals human pluripotent stem cell-derived limbal epithelial stem cells are similar to native ocular surface epithelial cells.
Mikhailova, A; Jylhä, A; Rieck, J; Nättinen, J; Ilmarinen, T; Veréb, Z; Aapola, U; Beuerman, R; Petrovski, G; Uusitalo, H; Skottman, H
Scientific reports  5  14684  2015

Show Abstract
26423138 26423138
Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
Saghizadeh, M; Epifantseva, I; Hemmati, DM; Ghiam, CA; Brunken, WJ; Ljubimov, AV
Investigative ophthalmology & visual science  54  8172-80  2013

Show Abstract
24255036 24255036
Adenosine triphosphate-binding cassette transporter G2 expression in endometriosis and in endometrium from patients with and without endometriosis.
Matsuzaki, Sachiko and Darcha, Claude
Fertil. Steril., 98: 1512-1520.e3 (2012)  2012

Show Abstract
22925688 22925688
Epidermal differentiation and loss of clonal growth potential of human limbal basal epithelial progenitor cells during intrastromal invasion.
Tan, EK; He, H; Tseng, SC
Investigative ophthalmology & visual science  52  4534-45  2011

Show Abstract
21527382 21527382
Differences between human and mouse embryonic stem cells.
Ginis, Irene, et al.
Dev. Biol., 269: 360-80 (2004)  2004

15110706 15110706
Flow cytometry-based approach to ABCG2 function suggests that the transporter differentially handles the influx and efflux of drugs.
Marta García-Escarp, Vanessa Martínez-Muñoz, Irene Sales-Pardo, Jordi Barquinero, Joan Carles Domingo, Pedro Marin, Jordi Petriz
Cytometry. Part A : the journal of the International Society for Analytical Cytology  62  129-38  2004

Show Abstract
15517563 15517563
Multidrug resistance mediated by the breast cancer resistance protein BCRP (ABCG2).
Doyle, L; Ross, DD
Oncogene  22  7340-58  2003

Show Abstract
14576842 14576842
The ABC transporter Brcp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side population phenotype
Zhou, S. et al.
Nat. Med., 7(9):1028-1034 (2001)  2001

11533706 11533706