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AB5490-I-100UL Anti-Atrial Natriuretic peptide

Anti-Atrial Natriuretic peptide, Cat. No. AB5490-I, is a highly specific rabbit polyclonal antibody, that targets Natriuretic peptides A and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

Anti-Atrial Natriuretic peptide MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Nppa UniProt Number: P01161
AB5490-I-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5490-I-100UL
Brand Family Chemicon®
DescriptionAnti-Atrial Natriuretic peptide
Alternate Names
  • CDD-ANF
  • Cardiodilatin
  • CDD
  • Cardiodilatin-related peptide
  • CDP
  • Prepronatriodilatin
Background InformationNatriuretic peptides A (UniProt: P01161; also known as CDD-ANF, ANP, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, Prepronatriodilatin) is encoded by the Nppa gene (Gene ID: 24062) in rat. Atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) are belong to the natriuretic peptide family. They are responsible for body fluid homeostasis and regulation of blood pressure. ANP is produced mainly by cardiac myocytes and is released in response to atrial stretch. Once in the circulation, ANP induces natriuresis, diuresis, and cause a reduction in blood pressure. In renal tissue ANP acts on receptors that increase glomerular filtration rate, reduce sodium reabsorption in the distal nephron, and diminish renin secretion. ANP has an intra-molecular ring structure connected by two cysteine residues and N-terminal and C-terminal extensions from it. ANP is produced with a signal peptide (aa 1-24) and a propeptide region (aa 25-122), which are cleaved to produce active hormone. It is cleaved by Corin by cleavage of Arg-Ser (aa 122-123) peptide bond. It can also be cleaved by membrane metalloendopeptidase between aa 129-130 that initiates its degradation and thereby regulate its activity.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Atrial Natriuretic peptide, Cat. No. AB5490-I, is a highly specific rabbit polyclonal antibody, that targets Natriuretic peptides A and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atrial Natriuretic peptide in rat heart and mouse heart tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal region of rat Natriuretic peptides A.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Natriuretic peptides A in rat and murine heart. It targets an epitope within 28 amino acids from the C-terminal region.
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nppa
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~17 kDa observed; 16.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat heart tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Atrial Natriuretic peptide in rat heart tissue lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5490-I-100UL 04054839460128