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MAB5372 Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31

Detect Astrocytomas using this Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 validated for use in WB, IC, IH(P).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers
MAB5372
100 µL  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Gp, H, M, RWB, ICC, IH(P)MAscitesMonoclonal Antibody
Description
Catalogue NumberMAB5372
ReplacesMAB5314
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31
References
Product Information
FormatAscites
PresentationAscites fluid. Liquid.
Quality LevelMQ100
Applications
ApplicationDetect Astrocytomas using this Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 validated for use in WB, IC, IH(P).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesWestern blot: reducing conditions, recognizes a 30kDa protein {Singh, R et al (1986) Biosci Rep 6(1):73-80.

Immunohistochemistry The antibody works on paraffin embedded tissue sections.

Immunocytochemistry

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman cerebral white matter plaque materials from a multiple sclerosis patient.
Epitopea marker for Low Grade Astrocytoma
CloneJ1-31
HostMouse
SpecificityThe antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed).

Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}.
IsotypeIgM
Species Reactivity
  • Guinea Pig
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB5372 04053252464003

Documentation

Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 SDS

Title

Safety Data Sheet (SDS) 

Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 Certificates of Analysis

TitleLot Number
MOUSE ANTI-ASTROCYTES (MARKER FOR LOW GRADE ASTROCYTOMA) MONOCLONAL ANTIBODY 3088366
MOUSE ANTI-ASTROCYTES (MARKER FOR LOW GRADE ASTROCYTOMA) MONOCLONAL ANTIBODY 2932383
MOUSE ANTI-ASTROCYTES -2621528 2621528
MOUSE ANTI-ASTROCYTES -2652173 2652173
MOUSE ANTI-ASTROCYTES -2714467 2714467
MOUSE ANTI-ASTROCYTES -2790072 2790072
MOUSE ANTI-ASTROCYTES -2815178 2815178

References

Reference overviewPub Med ID
Up-regulation of reactive astrogliosis in the rat glioma 9L cell line by combined mechanical and chemical injuries.
Malhotra, S K, et al.
Cytobios, 89: 115-34 (1997)  1997

9363621 9363621
Diversity among reactive astrocytes: proximal reactive astrocytes in lacerated spinal cord preferentially react with monoclonal antibody J1-31.
Malhotra, S K, et al.
Brain Res. Bull., 30: 395-404 (1993)  1993

8457889 8457889
Novel astrocytic protein in multiple sclerosis plaques.
Malhotra, S K, et al.
J. Neurosci. Res., 22: 36-49 (1989)  1989

2926840 2926840
Enhanced expression of a protein antigen (J1-31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord.
Predy, R, et al.
J. Neurosci. Res., 19: 397-404, 466-7 (1988)  1988

3385801 3385801
A new "marker" protein for astrocytes.
Singh, R, et al.
Biosci. Rep., 6: 73-80 (1986)  1986

2421799 2421799