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MABN2470-100UG Anti-Aquaporin-4 Antibody, clone mCCD

Anti-Aquaporin-4, clone mCCD Antibody, Cat. No. MABN2470, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-Aquaporin-4 Antibody, clone mCCD MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: AQP4 UniProt Number: P55087
MABN2470-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MICC, IHC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2470-100UG
DescriptionAnti-Aquaporin-4 Antibody, clone mCCD
Alternate Names
  • AQP-4
  • Mercurial-insensitive water channel
  • MIWC
  • WCH4
Background InformationAquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG3 in PBS without preservatives.
Applications
ApplicationAnti-Aquaporin-4, clone mCCD Antibody, Cat. No. MABN2470, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).
Biological Information
ImmunogenHis-tagged full length human recombinant Aquaporin-4.
Epitopecytoplasmic domain
ClonemCCD
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.
IsotypeIgG3κ
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Rat, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • AQP4
Purification MethodProtein G purified
UniProt Number
Molecular Weight34.83 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in rat brain frozen tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Aquaporin-4 in rat brain frozen tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2470-100UG 04054839551031