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AP1031 Anti-Apollon (4775-4829) Rabbit pAb

Anti-Apollon (4775-4829) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-BIRC6, Anti-Baculoviral IAP repeat-containing 6, Anti-BRUCE, Anti-KIAA1289

AP1031
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~500 kDa Apollon protein in HeLa cells.
Catalogue NumberAP1031
Brand Family Calbiochem®
SynonymsAnti-BIRC6, Anti-Baculoviral IAP repeat-containing 6, Anti-BRUCE, Anti-KIAA1289
Application Data
Detection of human Apollon by immunoprecipitation and immunoblotting. Samples: Whole cell lysate (20 μg, lane 1, 50 μg, lane 2) from HeLa cells or S100 extract (20 μg, lane 3, 50 μg, lane 4) from HeLa cells. Antibody for IP: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (2 μg/mg lysate protein). Antibody for IB: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (1 μg/ml). Detection: chemiluminescence.
References
ReferencesQiu, X.B., et al. 2005. J. Biol. Chem. 280, 174.
Ren, J., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 565.
Hao, Y., et al. 2004. Nat. Cell Biol. 6, 849.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
Positive controlHeLa whole cell lysate or HeLa cell S100 extract
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunoprecipitation (2 µg/mg lysate protein)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 4775-4829 of human BIRC6/Apollon
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1031 0

Documentation

Anti-Apollon (4775-4829) Rabbit pAb Certificates of Analysis

TitleLot Number
AP1031

References

Reference overview
Qiu, X.B., et al. 2005. J. Biol. Chem. 280, 174.
Ren, J., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 565.
Hao, Y., et al. 2004. Nat. Cell Biol. 6, 849.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-BIRC6, Anti-Baculoviral IAP repeat-containing 6, Anti-BRUCE, Anti-KIAA1289
ApplicationImmunoblotting (1 µg/ml)
Immunoprecipitation (2 µg/mg lysate protein)
Application Data
Detection of human Apollon by immunoprecipitation and immunoblotting. Samples: Whole cell lysate (20 μg, lane 1, 50 μg, lane 2) from HeLa cells or S100 extract (20 μg, lane 3, 50 μg, lane 4) from HeLa cells. Antibody for IP: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (2 μg/mg lysate protein). Antibody for IB: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (1 μg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~500 kDa Apollon protein.
BackgroundApollon (Q9NR09) is a ~500 kDa membrane associated inhibitor of apoptosis (IAP) protein. It encodes baculoviral-IAP-repeat (BIR) and ubiquitin-conjugating enzyme (UBC) domains at the N and C-termini, respectively. Unlike other IAPs Apollon encodes a chimeric E2/E3 ubiquitin ligase and is essential for inhibition of apoptosis in certain cell lines. Apollon inhibits apoptosis by promoting ubiquitination and degradation of active Smac and caspase-9, but not caspase-3. Unlike other IAPs, Apollon also binds to and inactivates precursors of caspase-9 and Smac. Inactivation of Apollon also leads to upregulation and nuclear localization of p53, which results in activation of caspase-2, Bax, and Bak, ultimately leading to mitochondrial apoptosis.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 4775-4829 of human BIRC6/Apollon
IsotypeIgG
Specieshuman
Positive controlHeLa whole cell lysate or HeLa cell S100 extract
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Toxicity Standard Handling
ReferencesQiu, X.B., et al. 2005. J. Biol. Chem. 280, 174.
Ren, J., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 565.
Hao, Y., et al. 2004. Nat. Cell Biol. 6, 849.