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MAB2605 Anti-ATG5 Antibody, clone 177.19

This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.

Anti-ATG5 Antibody, clone 177.19 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: APG5 , APG5-LIKE , APG5L, ASP, hAPG5 UniProt Number: Q9H1Y0
MAB2605
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2605
ReplacesMABC137
DescriptionAnti-ATG5 Antibody, clone 177.19
Alternate Names
  • APG5 autophagy 5-like
  • APG5 autophagy 5-like (S. cerevisiae)
  • APG5 (autophagy 5, S. cerevisiae)-like
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
  • ATG5 autophagy related 5-like
  • Apoptosis-specific protein
Background InformationATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity
References
Product Information
FormatPurified
Control
  • Western Blot and Immunoprecipitation: HeLa cell lysate.
PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Quality LevelMQ100
Applications
ApplicationThis Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation: HeLa cell lysate
Biological Information
ImmunogenLinear Peptide, aa: 8-318
Clone177.19
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThe antibody reacts with the 50 kDa ATG5 protein.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteReacts with human. Other species have not been tested.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APG5
  • APG5-LIKE
  • APG5L
  • ASP
  • hAPG5
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed By similarity.
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
SUBCELLULAR LOCATION: Cytoplasm. Note= Colocalizes with nonmuscle actin.
TISSUE SPECIFICTY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
INDUCTION: By apoptotic stimuli.
PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
SEQUENCE SIMILARITIES: Belongs to the ATG5 family
Molecular Weight50 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceWestern Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB2605 04053252398391