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PC285 Anti-AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb

Anti-AML1/RHD Domain (Ab-2) (50-177), rabbit polyclonal, recognizes the ~58 kDa AML1 protein in overexpressing cells such as in Jurkat and MOLT-4 cells. It is validated for Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Acute Myeloid Leukemia 1, Anti-Runx1

PC285
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~58 kDa AML1 protein in overexpressing cells such as Jurkat and MOLT-4 cells.
Catalogue NumberPC285
Brand Family Calbiochem®
SynonymsAnti-Acute Myeloid Leukemia 1, Anti-Runx1
References
ReferencesStrom, D.K., et al. 2000. J. Biol. Chem. 275, 3438.
Lo Coco, F., et al. 1997. Haematologica 82, 364.
Mitani, K., 1997. Leukemia 11 (Suppl 3) 503.
Mitani, K., 1997. Leukemia 11 (Suppl 3) 294.
Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
Hiebert, S.W., et al. Mol. Cell Biol. 16, 1349.
Kurokawa, M., et al. 1996. J. Biol. Chem. 271, 16870.
Sacchi, N., et al. 1996. Oncogene 12, 437.
Meyers, S., et al. 1995. Mol. Cell. Biol. 15, 1974.
Miyoshi, H., et al. 1995. Nuclei Acids Res. 23, 2762.
Levanon, D., et al. 1994. Genomics 23, 425.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlJurkat or MOLT-4 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Not Frozen Sections
Not Immunofluorescence
Not Immunoprecipitation
Not Paraffin Sections
Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Paraffin Sections (not recommended)
Application CommentsThis antibody preferentially reacts with the runt homology domain (RHD) on AML1. Reactivity with other AML family members is negligible. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 50-177 of human AML1
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC285 0

Documentation

Anti-AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb Certificates of Analysis

TitleLot Number
PC285

References

Reference overview
Strom, D.K., et al. 2000. J. Biol. Chem. 275, 3438.
Lo Coco, F., et al. 1997. Haematologica 82, 364.
Mitani, K., 1997. Leukemia 11 (Suppl 3) 503.
Mitani, K., 1997. Leukemia 11 (Suppl 3) 294.
Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
Hiebert, S.W., et al. Mol. Cell Biol. 16, 1349.
Kurokawa, M., et al. 1996. J. Biol. Chem. 271, 16870.
Sacchi, N., et al. 1996. Oncogene 12, 437.
Meyers, S., et al. 1995. Mol. Cell. Biol. 15, 1974.
Miyoshi, H., et al. 1995. Nuclei Acids Res. 23, 2762.
Levanon, D., et al. 1994. Genomics 23, 425.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-August-2008 JSW
SynonymsAnti-Acute Myeloid Leukemia 1, Anti-Runx1
ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Paraffin Sections (not recommended)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~58 kDa AML1 protein.
BackgroundThe family of nuclear transcription factors represented by AML are important transactivators of tissue-specific genes of the hematopoietic lineage and the bone cell lineage. There are currently three different mammalian proteins that are members of this family, AML1, AML2, and AML3. The AML proteins in turn form heterodimers with another protein, CBFbeta and it is this complex which has the transcription factor activity. Within the complex the AML protein provides the DNA binding function. The AML gene was discovered in 1991 by cloning the t(8;21) translocation that is common to AML. Structurally the AML protein contains a transactivation domain at the C-terminus and has homology with the Drosophila Runt gene that is the DNA binding protein of the protein. In leukemias, AML1 undergoes a translocation and becomes fused to MTG8 (ETO) and forms a chimeric protein (83 kDa). This chimera is in turn complexed with the CBFbeta gene product that also has undergone a translocation. In addition to MTG8, AML1 has also been shown to undergo translocation with TEL, and EVI. Splice variants have been reported for AML which include AML1a (30 kDa), AML1b (54 kDa), and AML1c. All forms contain the runt domain but vary in their transactivation ability. Additionally 6 major transcripts have been shown for AML due to the use of 2 promoters and differential use of 3 polyadenylation sites in addition to the splice variants.
HostRabbit
Immunogen speciesHuman
Immunogena recombinant protein consisting of amino acids 50-177 of human AML1
IsotypeIgG
Specieshuman, mouse
Positive controlJurkat or MOLT-4 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody preferentially reacts with the runt homology domain (RHD) on AML1. Reactivity with other AML family members is negligible. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesStrom, D.K., et al. 2000. J. Biol. Chem. 275, 3438.
Lo Coco, F., et al. 1997. Haematologica 82, 364.
Mitani, K., 1997. Leukemia 11 (Suppl 3) 503.
Mitani, K., 1997. Leukemia 11 (Suppl 3) 294.
Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
Hiebert, S.W., et al. Mol. Cell Biol. 16, 1349.
Kurokawa, M., et al. 1996. J. Biol. Chem. 271, 16870.
Sacchi, N., et al. 1996. Oncogene 12, 437.
Meyers, S., et al. 1995. Mol. Cell. Biol. 15, 1974.
Miyoshi, H., et al. 1995. Nuclei Acids Res. 23, 2762.
Levanon, D., et al. 1994. Genomics 23, 425.