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AM63 Anti-A20 Mouse mAb (59A426)

Anti-A20 mouse monoclonal antibody, clone 59A426, recognizes ~70 kDa A20 protein in Daudi cells. It is validated for use in immunoblotting and immunoprecipitation.

Anti-A20 Mouse mAb (59A426) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
AM63
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AM63-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~70 kDa A20 protein in Daudi cells.
Catalogue NumberAM63
Brand Family Calbiochem®
Application Data
Detection of human A20 by immunoblotting. Sample: Whole cell lysate (10 µg) from Daudi cells. Primary antibody: Anti-A20 Mouse mAb (59A426) (Cat. No. AM63) (3 µg/ml). Detection: chemiluminescence.
References
ReferencesHeyninck, K., et al. J. Cell Biol 145, 1471.
May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63.
Tewari, M., et al. 1995. J. Immunol. 154, 1699.
Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.
Product Information
FormLiquid
FormulationIn PBS, 0.2% gelatin.
Positive controlDaudi cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:500-1:2000)
Immunoprecipitation (2 µg/mg)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenfull-length recombinant human A20 protein
ImmunogenHuman
Clone59A426
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AM63-100UG 04055977228021

Documentation

Anti-A20 Mouse mAb (59A426) SDS

Title

Safety Data Sheet (SDS) 

Anti-A20 Mouse mAb (59A426) Certificates of Analysis

TitleLot Number
AM63

References

Reference overview
Heyninck, K., et al. J. Cell Biol 145, 1471.
May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63.
Tewari, M., et al. 1995. J. Immunol. 154, 1699.
Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-August-2007 RFH
ApplicationImmunoblotting (1:500-1:2000)
Immunoprecipitation (2 µg/mg)
Application Data
Detection of human A20 by immunoblotting. Sample: Whole cell lysate (10 µg) from Daudi cells. Primary antibody: Anti-A20 Mouse mAb (59A426) (Cat. No. AM63) (3 µg/ml). Detection: chemiluminescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~70 kDa A20 protein.
BackgroundNF-κB (nuclear factor κB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-κB gene expression by interferring with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IκB kinase pathway. A20, an 70 kDa protein, is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues.
HostMouse
Immunogen speciesHuman
Immunogenfull-length recombinant human A20 protein
Clone59A426
IsotypeIgG₁
Specieshuman
Positive controlDaudi cells
FormLiquid
FormulationIn PBS, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot freeze (-20°C).
Toxicity Standard Handling
ReferencesHeyninck, K., et al. J. Cell Biol 145, 1471.
May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63.
Tewari, M., et al. 1995. J. Immunol. 154, 1699.
Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.