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ST1049 Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subuni

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ST1049
  
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, Rb, R, YeastMMonoclonal Antibody
Description
OverviewRecognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
Catalogue NumberST1049
Brand Family Calbiochem®
Application Data
Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
References
ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHuman placental tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Not Immunoprecipitation
Application NotesImmunoblotting (1:1000, chemiluminescence)
Immunoprecipitation (not recommended)
ELISA (see comments)
Immunofluorescence (see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Frozen Sections (see application references)
Application CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogendinitrophenylated proteasomes
ImmunogenHuman
Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
CloneMCP231
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rabbit
  • Rat
  • Yeast
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
ST1049 0