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SCR140 Animal Free Collagenase/Dispase Blend II

Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution

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Animal Free Collagenase/Dispase Blend II MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Harvesting, Cell Culture
SCR140
50mg  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key ApplicationsSource
Harv, CULTIsolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2015 certified enviroment.
Description
Catalogue NumberSCR140
DescriptionAnimal Free Collagenase/Dispase Blend II
Alternate Names
  • MMP
  • Matrix metalloprotease
  • Dispase
  • Neutral Protease
Background InformationCrude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. EMD Millipore’s Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. The product is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.
Product Information
PresentationProduct is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.
ActivityCollagenase Activity: ≥12,500 units per vial
Caseinase Activity: ≥2000 caseinase units/mg
Clostripain Activity: ≤5.0 clostripain units/mg
Tryptic Activity: ≤0.5 trypic units/mg
Quality LevelMQ100
Applications
ApplicationAnimal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
Key Applications
  • Cell Harvesting
  • Cell Culture
Biological Information
SourceIsolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2015 certified enviroment.
Physicochemical Information
ActivityCollagenase Activity: ≥12,500 units per vial
Caseinase Activity: ≥2000 caseinase units/mg
Clostripain Activity: ≤5.0 clostripain units/mg
Tryptic Activity: ≤0.5 trypic units/mg
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
Packaging Information
Material Size50mg
Global Trade Item Number
Catalogue Number GTIN
SCR140 04053252982699