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175600 Angiogenin, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
175600
  
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Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE
Description
OverviewRecombinant, human angiogenin expressed in E. coli. Exhibits angiogenic and ribonucleolytic activities. Enhances the ability of endothelial cells to digest extracellular matrix components and degrade basement membrane. Has been shown to stimulate capillary and umbilical vein endothelial cells to produce DAG and secrete prostacyclin by phospholipase activation. Biological activity: 1.0 µg protein produces an absorbance change at 260 nm of approximately 2.0 - 3.0 as measured by yeast tRNA ribonucleolytic activity.
Catalogue Number175600
Brand Family Calbiochem®
References
ReferencesLeland, P.A., et al. 2002. Biochemistry 41, 1343.
Hatzi, E., et al. 2000. Biochem. Biophys. Res. Commun. 267, 719.
Hu, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 12096.
Lee, F.S., and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161, 121.
Kurachi, R., et al. 1985. Biochemistry 24, 5494.
Product Information
FormSolid
FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg Angiogenin.
Quality LevelMQ100
Applications
Biological Information
Biological activity≥1.0 µg protein produces an absorbance change at 260 nm of ~2.0-3.0 as measured based upon its ribonucleolytic activity toward Yeast tRNA.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxins: ≤100 pg/µg Angiogenin
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to months at -70°C. Avoid freeze/thaw cycles of solutions.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
175600 0

Documentation

Angiogenin, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Angiogenin, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
175600

References

Reference overview
Leland, P.A., et al. 2002. Biochemistry 41, 1343.
Hatzi, E., et al. 2000. Biochem. Biophys. Res. Commun. 267, 719.
Hu, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 12096.
Lee, F.S., and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161, 121.
Kurachi, R., et al. 1985. Biochemistry 24, 5494.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-November-2009 JSW
DescriptionRecombinant, human angiogenin expressed in E. coli. A 14 kDa protein with angeogenic and ribonucleolytic activities. Enhances the proteolytic and fibrinolytic activities of endothelial cells. Also, stimulates the endothelial cell invasion of Matrigel basement membrane. Has been shown to stimulate capillary and umbilical vein endothelial cells to produce DAG and secrete prostacyclin by phospholipase activation.
FormSolid
FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg Angiogenin.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxins: ≤100 pg/µg Angiogenin
Biological activity≥1.0 µg protein produces an absorbance change at 260 nm of ~2.0-3.0 as measured based upon its ribonucleolytic activity toward Yeast tRNA.
SolubilityReconstitute to a concentration ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesLeland, P.A., et al. 2002. Biochemistry 41, 1343.
Hatzi, E., et al. 2000. Biochem. Biophys. Res. Commun. 267, 719.
Hu, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 12096.
Lee, F.S., and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161, 121.
Kurachi, R., et al. 1985. Biochemistry 24, 5494.