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UFC503096 Amicon Ultra-0.5 Centrifugal Filter Unit

Ultracel-30 regenerated cellulose membrane, 0.5 mL sample volume

Key Applications: Protein Extraction, Protein Purification
UFC503096
96  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsNMWLVolumeMinimum Final Concentrate Volume
PExt, Protein Purification30 KDa0.5 mL15 µL
Description
Catalogue NumberUFC503096
Replaces42410
Trade Name
  • Amicon Ultra
DescriptionAmicon Ultra-0.5 Centrifugal Filter Unit
Background InformationAMICON ULTRA 0.5ML - 30KDa cutoff. Available in 4 pack sizes.
UFC503008 - 8 pack
UFC503024 - 24 pack
UFC503096 - 96 pack
UFC5030BK - 500 pack


Concentrates 500 µL down to 15 µL
Concentration factor 25 x to 30 x
High sample recovery (greater than 90% of dilute starting solution)
Fast processing time - 10 to 30 minutes
Reverse spin capability provides consistent recoveries

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates
Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
Desalting, buffer exchange and protein dialysis

Material Supplied:
The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

Performance – DNA Concentration:
The Amicon Ultra-0.5 device provides the best balance between recovery and spin time for double-stranded DNA for base pairs ranging from 137 to 1159. To achieve maximum PCR product recovery and primer removal with primers greater than 20 bases, one or two additional spins with Tris-EDTA (TE) buffer are recommended.
References
Product Information
Filter CodePLCGC
Color CodeClear
Filter TypeUltrafiltration
NMWL30 KDa
Quality LevelMQ400
Applications
ApplicationUltracel-30 regenerated cellulose membrane, 0.5 mL sample volume
Key Applications
  • Protein Extraction
  • Protein Purification
Biological Information
SterilityNon-Sterile
Physicochemical Information
Centrifugal ApplicationProtein Concentration and Desalting
Dimensions
Length4.99 cm (1.96 in.)
Diameter10.8 mm
Filtration Area1 cm²
Minimum Final Concentrate Volume15 µL
Volume0.5 mL
Materials Information
Chemistry
  • Regenerated Cellulose (RC)
Device Material
  • Housing: Styrene/butadiene
  • Cap and Tube: Polypropylene
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size96
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
UFC503096 04053252464591

Documentation

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Categories

Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units