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05-1050 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

05-1050
100 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ICC, ELISA, IHC, IF, FC, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1050
Brand Family Upstate
Trade Name
  • Upstate
Description4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
Background InformationThe development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
Product Information
FormatPurified
Control
  • Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
PresentationProprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide.
Liquid at 2-8°C.
Quality LevelMQ100
Applications
Application4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • ELISA
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Immunoprecipitation
Application NotesImmunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Biological Information
Immunogen4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
Clone4G10®
HostMouse
SpecificityTyrosine-phosphorylated proteins from all species
IsotypeIgG2b
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G-Sepharose™ Chromatography
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
05-1050 04053252677236