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L1613-BC 1 M HEPES Buffer (50x)

A 1 M solution of HEPES that provides good buffering in the pH range of 7.2 to 7.6.

L1613-BC
  
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Overview

Replacement Information
Description
OverviewHEPES (hydroxyethyl-piperazineethane-sulfonic acid buffer) is an organic buffer and very stable. In the physiological area HEPES has an excellent buffer capacity in a pH range of 7.2 to 7.6. HEPES is apt for all cell culture systems with a high proliferation and cell density. On HEPES buffered cell culture media, the incubation in a CO2 incubator is not necessary.

Specifications:

Sterility: Pass
pH: 7.35-7.55
Osmolality: 1.65-2.00 Osm/kg H20
Particles/ml (>10µM): < 25/ml
Particles/ml (>25µM): < 3/ml
Color: Colorless
Appearance: Clear

How to use HEPES buffer:

• dissolve powder and adjust pH at 7.2 with sodium hydroxide
• add 10-20 ml of HEPES buffer per litre of medium (for very delicate cell cultures, use only 10 ml)
• a concentration of 20 mM HEPES requires 20 ml/l medium, for 25 mM use 25 ml/l
• if NaHCO3 is required, only add 10%
Catalogue NumberL1613-BC
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Storage +2°C to +25°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
L1613-BC 0

Documentation