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407320 γ-Interferon, Mouse, Recombinant, E. coli

407320
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
407320-100UG Plastic ampoule 100 μg
407320-20UG Plastic ampoule 20 μg
Description
OverviewRecombinant, mouse γ-interferon (γ-IFN) expressed in E. coli as a single, non-glycosylated polypeptide containing 134 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
Catalogue Number407320
Brand Family Calbiochem®
SynonymsInterferon γ precursor, IFN-γ, rmIFN-γ
References
ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
Product Information
FormLyophilized
FormulationLyophilized from 0.5X PBS, pH 7.2.
Quality LevelMQ100
Applications
Biological Information
Biological activityReported as IU/mg on a lot-specific basis using a cytopathic effect inhibition assay.
Purity≥95% by RP-HPLC, FPLC, and SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
407320-100UG 04055977188318
407320-20UG 04055977188325

Documentation

γ-Interferon, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

γ-Interferon, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407320

References

Reference overview
Alvarado, J., et al. 2005. Immunol. Lett. 99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-March-2009 JSW
SynonymsInterferon γ precursor, IFN-γ, rmIFN-γ
DescriptionRecombinant, mouse γ-interferon (γ-IFN) expressed in E. coli as a single, non-glycosylated polypeptide containing 134 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
FormLyophilized
FormulationLyophilized from 0.5X PBS, pH 7.2.
Purity≥95% by RP-HPLC, FPLC, and SDS-PAGE
Biological activityReported as IU/mg on a lot-specific basis using a cytopathic effect inhibition assay.
SolubilityReconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.
Storage Protect from moisture
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.