Millipore Sigma Vibrant Logo

109253 Papanicolaou's solution 1a Harris' hematoxylin solution

for cytology

Papanicolaou's solution 1a Harris' hematoxylin solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Hematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution

View Products on Sigmaaldrich.com
109253
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1092530500
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic bottle 500 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      Catalogue Number109253
      SynonymsHematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution
      DescriptionPapanicolaou’s solution 1a Harris hematoxylin solution
      OverviewPapanicolaou’s solution 1a Harris hematoxylin solution - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by (Papanicolaou’s solution 2b Orange II solution, (Cat. no. 1106887) or Papanicolaou’s solution 2a Orange G solution (OG6), (Cat. no. 106888)), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat.no. 109254) give a blue to dark violet staining result with clinical specimen material.
      A 500 ml bottle of either solution is sufficient for 1500 -2500 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      HS Code3212 90 00
      Quality LevelMQ400
      Applications
      Applicationfor cytology
      Biological Information
      Physicochemical Information
      Boiling point100 °C (1013 hPa)
      Density1.04 g/cm3 (20 °C)
      pH value2.5 - 3.0 (H₂O, 20 °C)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Statement(s)H318: Causes serious eye damage.
      EUH210: Safety data sheet available on request.
      Precautionary Statement(s)P280: Wear eye protection/ face protection.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      Signal WordDanger
      Storage class10 - 13 Other liquids and solids
      WGKWGK 1 slightly hazardous to water
      Disposal28
      Aqueous solutions: Container D.
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Suitability for microscopy (Vaginal smear)passes test
      Nucleiblue to dark violet
      Cyanophilic Cytoplasm (basophilic)blue-green
      Eosinophilic Cytoplasm (acidophilic)pink
      Global Trade Item Number
      Catalogue Number GTIN
      1092530500 04022536112466

      Documentation

      Papanicolaou's solution 1a Harris' hematoxylin solution SDS

      Title

      Safety Data Sheet (SDS) 

      Papanicolaou's solution 1a Harris' hematoxylin solution Certificates of Analysis

      Product Number PackagingSpecificationLot Number
      1092530500 Plastic bottlePDF Specification Document

      User Guides

      Title
      109253 Solución de Papanicolaou 1a solución de hematoxilina según Harris para citología
      109253 Solución de Papanicolaou 1a solución de hematoxilina según Harris para citología (versión de Cuba)
      User Guide (IFU) - 109253

      Related Products & Applications

      Accessories (Primary)

      Catalogue Number Description  
      106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
      109272 Papanicolaou's solution 3b polychromatic solution EA 50 Show Pricing & Availability

      Accessories (Secondary)

      Catalogue Number Description  
      104302 Hematoxylin cryst. (C.I. 75290) Show Pricing & Availability
      106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
      109272 Papanicolaou's solution 3b polychromatic solution EA 50 Show Pricing & Availability

      Related Products By: Application Facete

      Clinical Cytology
      Cytodiagnosis
      Papanicolaou

      Categories

      IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents