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112084 HEMATOGNOST Fe™

staining kit for the detection of free ionic iron (Fe³⁺) in cells

HEMATOGNOST Fe™ MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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112084
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      		 Fibre case 4 x 250 ml
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      Description
      Catalogue Number112084
      DescriptionHEMATOGNOST Fe™
      OverviewHEMATOGNOST Fe™ - staining kit for the detection of free ionic iron (Fe3+) in cells, is used for human-medical cell diagnosis and serves the purpose of the hematological and histological investigation of sample material of human origin. HEMATOGNOST Fe™ is a staining kit for performing the so-called Prussian blue (Berlin blue) reaction in hematological and histological specimen material. This staining kit contains all the reagents necessary for the detection of free ionic iron. In the Prussian blue reaction, ionic iron (Fe3+) not bound to the heme structure reacts with potassium hexacyanoferrate(II) in hydrochloric solution. It precipitates as an insoluble complex salt in the blood, bone-marrow, or tissue cells and thus localizes free cellular iron: 4 Fe3+ + 3 K4Fe(CN)6 = Fe4[Fe(CN)6]3 + 12 K+
      As a measure to achieve the best possible visual differentiation of the iron deposits in the cytoplasma, this is counterstained with nuclear fast red solution (Nuclear fast red-aluminum sulfate solution 0.1% (Cat. no.100121), resulting in a tender pink color. If further, more detailed information on the cell morphology is required, counterstaining with any of the other familiar “classical” methods such as Giemsa, (Giemsa, Cat. no. 109204), May-Grünwald (May-Grünwald eosin methylene blue solution, Cat.no 101424), hemalum (Mayer's hemalum solution, Cat. no.109249), etc. can also be employed.
      The Prussian blue reaction can also be used to stain specimens already stained by one of these classical procedures for the additional detection of iron. Repeat counterstaining is not necessary in this case.
      The staining kit is sufficient for 6 - 8 stainings with up to 16 slides. The staining kit is developed and registered as CE / IVD product for applications in diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      HS Code3212 90 90
      Quality LevelMQ400
      Applications
      Applicationstaining kit for the detection of free ionic iron (Fe³⁺) in cells
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Statement(s)H290: May be corrosive to metals.
      H318: Causes serious eye damage.
      Precautionary Statement(s)P234: Keep only in original packaging.
      P280: Wear eye protection/ face protection.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P390: Absorb spillage to prevent material damage.
      Signal WordDanger
      Storage class8B Non-combustible, corrosive hazardous materials
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 3316 Chemie-Testsatz, 9, II
      Declaration (transport by air) IATA-DGRUN 3316 CHEMICAL KIT, 9, II
      Declaration (transport by sea) IMDG-CodeUN 3316 CHEMICAL KIT, 9, II
      Supplemental Information
      Specifications
      Potassium hexacyanoferrate II (Reagent 1)4.5 - 5.5 %
      Hydrochloric acid (Reagent 2)4.5 - 5.5 %
      Density (Reagent 3 d 20 °C/4 °C)1.040 - 1.060
      Suitability for microscopypasses test
      Global Trade Item Number
      Catalogue Number GTIN
      1120840001 04022536139234

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      Categories

      IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Kits
      IVD/OEM Materials & Reagents > Microscopy > Hematology > Cytochemical Reagents