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110218 Gram's decolorizing solution

for the Gram staining method

Gram's decolorizing solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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110218
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Overview

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      		 Plastic bottle 500 ml
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      Description
      Catalogue Number110218
      DescriptionGram's decolorizing solution
      OverviewGram’s decolorizing solution - for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution (Gram's safranine solution (Cat.no. 109217) and appear pink to red.
      A bottle with 500 ml Gram's decolorizing solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no. 111885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU).
      References
      Product Information
      HS Code3814 00 90
      Quality LevelMQ400
      Applications
      Applicationfor the Gram staining method
      Biological Information
      Physicochemical Information
      Density0.79 g/cm3 (20 °C)
      Flash point-10 °C
      Ignition temperature>425 °C
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H225: Highly flammable liquid and vapour.
      H319: Causes serious eye irritation.
      H336: May cause drowsiness or dizziness.
      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
      P233: Keep container tightly closed.
      P240: Ground and bond container and receiving equipment.
      P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
      P242: Use non-sparking tools.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
      Signal WordDanger
      Storage class3 Flammable liquids
      WGKWGK 1 slightly hazardous to water
      Disposal1
      Strongly contaminated halogen-free organic solvents: container A.
      Safety Information
      Categories of dangerhighly flammable, irritant
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 1993 , 3, II
      Declaration (transport by air) IATA-DGRUN 1993 , 3, II
      Declaration (transport by sea) IMDG-CodeUN 1993 , 3, II
      Supplemental Information
      Specifications
      Suitability for microscopypasses test
      Staphylococcus aureusdark blue-violet
      E.colipink to red
      Global Trade Item Number
      Catalogue Number GTIN
      1102180500 04022536122663

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