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109204 Giemsa's azur eosin methylene blue solution

for microscopy

Giemsa's azur eosin methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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109204
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Overview

Replacement Information

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      		 Plastic bottle 500 ml
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      1092041000
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          		 Plastic bottle 1 l
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          Description
          Catalogue Number109204
          DescriptionGiemsa's azur-eosin-methylene blue solution
          OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor microscopy
          Biological Information
          Physicochemical Information
          Boiling point>65 °C (1013 hPa)
          Density0.99 g/cm3 (20 °C)
          Explosion limit5.5 - 44 %(V) Methanol
          Flash point18 °C
          Ignition temperature455 °C
          pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Pictogram(s)
          Hazard Pictogram(s)
          Hazard Statement(s)H225: Highly flammable liquid and vapour.
          H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
          H317: May cause an allergic skin reaction.
          H370: Causes damage to organs.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P233: Keep container tightly closed.
          P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
          P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
          P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
          P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
          P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
          Signal WordDanger
          Storage class3 Flammable liquids
          WGKWGK 2 obviously hazardous to water
          Disposal9
          Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
          Safety Information
          Categories of dangerhighly flammable, toxic
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
          Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
          Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
          Supplemental Information
          Specifications
          Suitability for microscopypasses test
          Erythrocytesreddish-brownish
          Nuclei / Chromatinred to violet
          Eosinophilic granulesreddish to red-brown
          Neutrophilic granuleslight violet
          Lymphocyte cytoplasmnblue
          Monocyte cytoplasmgrey-blue
          Thrombocytesviolet
          Basophilic granulesdark violet
          Nuclei (Tissue section)blue to dark blue
          Cellsblue to dark blue
          Collagenpale blue
          Osteoidpale blue
          Eosinophilic granula (Tissue section)red
          Acidic mucopolysaccharidesreddish-violet
          Mast cell granulesreddish-violet
          Cartilage matrixreddish-violet
          Acidophilic materialsorange-red
          Helicobacter pylori
          blue to dark blue
          blue to dark blue
          Global Trade Item Number
          Catalogue Number GTIN
          1092040500 04022536111889
          1092041000 04022536720081

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