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70933 pTriEx™-4 Neo DNA - Novagen

pTriEx™-4 Neo DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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70933
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70933-3CN 玻璃瓶 20 μg
Description
OverviewThe pTriEx™-4 Neo vector is designed to allow rapid characterization of target genes in E. coli, insect and vertebrate cells, and to allow rapid selection of stable transfected vertebrate cells expressing high levels of target gene. Expression in vertebrate cells is mediated by the CMV immediate early enhancer and promoter2. The drug selection marker is expressed under the control of the EMC virus Cap-Independent Translation Enhancer (CITE) sequence (or IRES), allowing rapid selection of transfected vertebrate cells using the drug G418 or neomycin sulfate. For expression in insect cells, pTriEx-4 Neo contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector® System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λlpL and λpI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows induction with IPTG. Native protein can be expressed by cloning into the Nco I site, or generated by cloning into the PshA I or Sma I sites and cleaving the fusion protein with enterokinase or thrombin, respectively.





This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70933
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70933-3CN 07790788060961

Documentation

pTriEx™-4 Neo DNA - Novagen MSDS

Title

Safety Data Sheet (SDS) 

pTriEx™-4 Neo DNA - Novagen Certificates of Analysis

TitleLot Number
70933

Citations

Title
  • Saurabh K. Gupta, et al. (2007) Human T-cell leukemia virus type1 tax oncoprotein prevents DNA damage-induced chromatin egress of hyperphosphorylated huChk2. Journal of Biological Chemistry 282, 29431-29440.
  • Gerard M. Gibbs, et al. (2006) The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. Journal of Biological Chemistry 281, 4156-4163.
  • Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.
  • Abdelhakim Ahmed-Belkacem, et al. (2005) Flavonoid structure-activity studies identify 6-prenylchrysin and tectochrysin as potent and specific inhibitors of breast cancer resistance protein ABCG2. Cancer Research 65, 4852-4860.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB250 pTriEx™ System Manual

    Vector Map

    Title
    TB299 pTriEx-4 Neo Vector Map

    Vector Sequence

    Title
    pTriEx-4 Neo Sequence

    Related Products & Applications

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    Categories

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits
    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Mammalian Expression > Mammalian Expression Vectors & Kits
    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors