Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-865 p53-GST (recombinant protein)

p53-GST (recombinant protein) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays UniProt Number: P04637 Entrez Gene Number: NP_000537
View Products on Sigmaaldrich.com
14-865
50 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

Special Shipping Offer on Antibodies
100% Performance Guaranteed
Description
Catalogue Number14-865
Brand Family Upstate
Trade Name
  • Upstate
Descriptionp53-GST (recombinant protein)
OverviewN-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography.
Alternate Names
  • transformation related protein 53
References
Product Information
PresentationRecombinant protein in buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 5mM glutathione, 0.5 mM EDTA, 0.5 mM DTT, 1 mM PMSF containing 20% glycerol.
Quality LevelMQ100
Applications
Key Applications
  • Enzyme Assays
Biological Information
Concentration0.5 mg/mL
Purity~95% by SDS-PAGE
SourceFull-length human p53 protein, expressed in e. coli.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]
Gene Symbol
  • p53
  • TP53
  • tumor protein p53
  • TRP53
  • FLJ92943
  • LFS1
Purification MethodGlutathione agarose chromatography
UniProt Number
Molecular Weight72 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by SDS-PAGE and Coomassie Stain
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-865 04053252516108

Documentation

p53-GST (recombinant protein) MSDS

Title

Safety Data Sheet (SDS) 

p53-GST (recombinant protein) Certificates of Analysis

TitleLot Number
p53-GST (recombinant protein expressed in E. coli) - 2394263 2394263
p53-GST (recombinant protein expressed in E. coli) 3042489
p53-GST (recombinant protein expressed in E. coli) - 2197248 2197248
p53-GST (recombinant protein expressed in E. coli) - 2273844 2273844
p53-GST (recombinant protein expressed in E. coli) - 2300093 2300093
p53-GST (recombinant protein expressed in E. coli) - 3282314 3282314
p53-GST (recombinant protein expressed in E. coli) - 3437416 3437416
p53-GST (recombinant protein expressed in E. coli) - 3447475 3447475
p53-GST - 1953008 1953008
p53-GST - 2049259 2049259
Show more

Related Products & Applications

Product Families

p53 Antibodies, Proteins & Kits

Millipore’s p53 antibodies demonstrate specificity against tumor antigen p53. See below for antibodies, kits, proteins and more for p53 research, based on the expertise of Upstate & Chemicon.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Other Proteins