Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GF154 Wnt-3a, recombinant mouse

The recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.

Wnt-3a, recombinant mouse MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P27467
View Products on Sigmaaldrich.com
GF154
5 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTMouse Mouse L cells expressing full length Wnt3a. Endotoxin level is less than 0.1ng/µg (1EU/µg).
Description
Catalogue NumberGF154
ReplacesGF160
DescriptionWnt-3a, recombinant mouse
OverviewRecombinant murine Wnt-3a is a monomeric glycoprotein containing 328 amino acid residues. When analyzed by SDS-PAGE under non-reducing conditions, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa.

Wnt-3a signalling has been implicated in the control of differentiation of stem cells. Wnt proteins bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. Wnt proteins have been shown to regulate cell-to-cell interactions during embryogenesis, more specifically regulating mesoderm differentiation and osteogenesis. Wnt proteins have also been shown to have putative roles in the regulation of adult stem cells.
References
Product Information
PresentationLyophilized from a solution containing PBS and 1.0% CHAPS buffer.
Quality LevelMQ100
Applications
ApplicationThe recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.
Key Applications
  • Cell Culture
Biological Information
PurityEndotoxin level is less than 0.1ng/µg (1EU/µg).
SourceMouse L cells expressing full length Wnt3a.
Specific ActivityWnt3a protein has been shown to be bioactive as determined by TCF/LEF-luciferase reporter. The ED50 for this effect is typically <250 ng/mL. Optimal concentrations should be determined for each application.
Gene Symbol
  • WNT3A
UniProt Number
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight38.0 - 41.0 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain the lyophilized material at -20°C up to six months from date of receipt. Resuspend in sterile water to a concentration of 100µg/mL. Reconstituted Wnt3a should be stored in single use aliquots at -80°C for up to three months. Avoid repeated freeze/thaw cycles. During shipment, some amount of product will occasionally dispersed. It’s recommended to gently tape the vial on the hard surface before reconstitution.
Packaging Information
Material Size5 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF154 04055977112344

Documentation

Wnt-3a, recombinant mouse MSDS

Title

Safety Data Sheet (SDS) 

Wnt-3a, recombinant mouse Certificates of Analysis

TitleLot Number
Wnt3a, Recombinant Mouse RD1403015
Wnt3a, Recombinant Mouse RD1403017
Wnt3a, Recombinant Mouse - 3191712 3191712
Wnt3a, Recombinant Mouse - 3526909 3526909
Wnt3a, Recombinant Mouse - 3615576 3615576
Wnt3a, Recombinant Mouse - 3864432 3864432
Wnt3a, Recombinant Mouse -2590159 2590159
Wnt3a, Recombinant Mouse -2797973 2797973
Wnt3a, Recombinant Mouse Recombinant Protein 2929723
Wnt3a, Recombinant Mouse Recombinant Protein 3067670
Show more