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14-811 UbcH5c Conjugating Enzyme, 100 µg

The His6-tagged fusion protein of UbcH5c is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.

UbcH5c Conjugating Enzyme, 100 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P61077
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14-811
100 µg  
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Overview

Replacement Information

Key Spec Table

Entrez Gene NumberSpeciesUni Prot Number
NM_003340.4 NM_181886.1 NM_181887.1 NM_181888.1 NM_181889.1 NM_181890.1 NM_181891.1 NM_181892.1 NM_181893.1 Human P61077
Description
Catalogue Number14-811
Brand Family Upstate
Trade Name
  • Upstate
DescriptionUbcH5c Conjugating Enzyme, 100 µg
OverviewThe His6-tagged fusion protein of UbcH5c is charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation. UbcH5c is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53, NF-κB, and c-Fos. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions.
References
Product Information
Quality LevelMQ100
Applications
ApplicationThe His6-tagged fusion protein of UbcH5c is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
Biological Information
Purity>95% by SDS-PAGE
SourceRecombinant human UbcH5c Conjugating Enzyme expressed in E. coli.
Entrez Gene Number
Entrez Gene SummaryThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
Gene Symbol
  • UBE2D3
  • UBCH5C
  • MGC43926
  • E2
  • UBC4/5
  • UbcH5C
  • E2(17)KB3
  • MGC5416
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P61077 # Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
SIZE: 147 amino acids; 16687 Da
SIMILARITY: SwissProt: P61077 ## Belongs to the ubiquitin-conjugating enzyme family.
Molecular Weight17 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -80°C from date of shipment. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-811 04053252516634

Documentation

UbcH5c Conjugating Enzyme, 100 µg MSDS

Title

Safety Data Sheet (SDS) 

UbcH5c Conjugating Enzyme, 100 µg Certificates of Analysis

TitleLot Number
UbcH5c Conjugating Enzyme (His6-tagged; human, recombinant protein) - 2141821 2141821
UbcH5c Conjugating Enzyme (His6-tagged; human, recombinant protein) - 2449247 2449247
UbcH5c Conjugating Enzyme (His6-tagged; human, recombinant protein) 3009993
UbcH5c Conjugating Enzyme - 0611046119 0611046119
UbcH5c Conjugating Enzyme - 2986004 2986004
UbcH5c Conjugating Enzyme - 3540382 3540382
UbcH5c Conjugating Enzyme - 4016242 4016242
UbcH5c Conjugating Enzyme - 4111203 4111203

References

Reference overviewPub Med ID
Enzymes catalyzing ubiquitination and proteolytic processing of the p105 precursor of nuclear factor kappaB1
Coux, O and Goldberg, A L
J Biol Chem, 273:8820-8 (1998)  1998

9535861 9535861
Identification of a family of closely related human ubiquitin conjugating enzymes
Jensen, J P, et al
J Biol Chem, 270:30408-14 (1995)  1995

8530467 8530467