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GF092 TRAIL Protein, Recombinant human

Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells.

TRAIL Protein, Recombinant human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P50591 Entrez Gene Number: NM_003810.2
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GF092
50 µg  
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Overview

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTHuman E.coliGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg of (1EU/μg) of TRAIL/Apo2L.
Description
Catalogue NumberGF092
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionTRAIL Protein, Recombinant human
OverviewHuman TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis, is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR transmembrane family of proteins and contain a cytoplasmic "death domain" which activates the cell's apoptotic machinery. GF092 is a 19.6 kDa protein comprising the full-length of the TNF-like extracellular domain of TRAIL.
Alternate Names
  • Apo2 Ligand
Product Information
PresentationSterile filtered, then lyophilized from 0.3 mg/mL in 10mM Sodium Phosphate + 110 mM NaCl.
Quality LevelMQ100
Applications
ApplicationHuman TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells.
Key Applications
  • Cell Culture
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg of (1EU/μg) of TRAIL/Apo2L.
SourceE.coli
Specific ActivityBIOLOGICAL ACTIVITY: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/mL.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
Gene Symbol
  • TNFSF10
  • TL2
  • Apo-2L
  • CD253
  • TRAIL-PEN
  • APO2L
  • TRAIL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P50591 # Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
COFACTOR: Binds 1 zinc ion per trimer.
SIZE: 281 amino acids; 32509 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein (Potential).
TISSUE SPECIFICITY: Widespread; most predominant in spleen, lung and prostate.
SIMILARITY: SwissProt: P50591 ## Belongs to the tumor necrosis factor family.
Stem Cell Type
  • Hematopoietic Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized protein may be stored at room temperature for a few weeks, but is best stored at -20°C. After a quick spin, reconstitute in sterile water to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted GF092 should be stored in undiluted aliquots at -20°C for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
GF092 04053252401756