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Buffer Detection Kit for Magnetic Beads
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PF133
Sigma-AldrichTACE, His•Tag®, Human, Recombinant, T. ni.
Recombinant, human TACE (amino acids 1-671) fused to a His•Tag® sequence and expressed in T. ni. insect cells. The major form of the purified protein corresponds to the mature form with an apparent molecular mass of ~70 kDa.
Catalogue Number
PF133
Brand Family
Calbiochem®
Synonyms
ADAM17, Human α-Secretase
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered 25 mM Tris-HCl, pH 8.0.
≥100 pmol/min/µg as measured by the cleavage of 10 µM of fluorescent substrate (MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂; Cat. No. 616402) in 100 µl of reaction buffer at 37°C.
Purity
≥90% by SDS-PAGE
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Avoid repeated freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
TACE, His•Tag®, Human, Recombinant, T. ni. Certificates of Analysis
Title
Lot Number
PF133
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-June-2008 RFH
Synonyms
ADAM17, Human α-Secretase
Description
Recombinant, human TACE (amino acids 1-671) His•Tag® sequence and expressed in T. ni. insect cells. The major form of the purified protein corresponds to the mature form with an apparent molecular mass of ~70 kDa.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered 25 mM Tris-HCl, pH 8.0.
Purity
≥90% by SDS-PAGE
Biological activity
≥100 pmol/min/µg as measured by the cleavage of 10 µM of fluorescent substrate (MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂; Cat. No. 616402) in 100 µl of reaction buffer at 37°C.
Solubility
Reconstitute with 100 µl dH₂O.
Preservative
None
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Avoid repeated freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
