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03-0168-00 SMC™ Human IL-5 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-5 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-5 in human serum and p

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Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
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03-0168-00
96 well assay  
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Overview

Key Spec Table

Species ReactivityKey Applications
NhP, Ca, R, MSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0168-00
Trade Name
  • SMC®
DescriptionSMC™ Human IL-5 High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human IL-5 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin 5 (IL-5), produced by T helper cells and mast cells, is a mediator of eosinophil maturation in bone marrow and release into the bloodstream and plays a role in eosinophil-based inflammatory processes, particularly allergic inflammation, asthma, and eosinophilic esophagitis. Inhibiting the action of IL-5 can suppress tissue damage due to eosinophil accumulation during pulmonary inflammation in asthma. As well, inhibition of IL-5 has been shown to reduce blood and bronchoalveolar eosinophilia caused by allergic challenge and chronic disease.
Product Information
Components
  • Assay Buffer
  • IL-5 Coated Beads
  • Standard Diluent
  • IL-5 Detection Antibody
  • IL-5 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <15%
  • Inter-assay CV% average (n=5): <20%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-5 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-5 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has been improved with the following features including new including new improvements over the legacy IL-5 kit, #03-0053-00:
• Improved LLOQ--0.04 pg/mL in the new streamlined kit while the legacy kit is 3.91 pg/mL (LLOQs of 0.01 pg/mL have been achieved with the new kit)
• Adjusted standard curve--changed to 0.01 – 50 pg/mL from 0.98 – 1000 pg/mL
• Added overnight incubation as an option for this assay
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
Biological Information
Species Reactivity
  • Non-human Primate
  • Canine
  • Rat
  • Mouse
Species Reactivity NoteAlthough IL-5 was detectable in non-human primate, canine, rat, and mouse samples, the assay has not been validated for these species.
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 98% with a range of 92-104%
  • Minimum of 5 plasma samples spiked with average recovery of 100% with a range of 95-106%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.04 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size96 well assay
Global Trade Item Number
Catalogue Number GTIN
03-0168-00 04054839695636