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557508 Ro-31-7549, Monohydrate - CAS 125313-65-7 - Calbiochem

A cell permeable, reversible, selective protein kinase C (PKC) inhibitor (IC₅₀ = 158 nM for rat brain PKC) that acts at the ATP binding site of PKC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 2-[1-3(Aminopropyl)indol-3-yl]-3(1-methyl-1H-indol-3-yl)maleimide, Acetate, Bisindolylmaleimide VIII, Acetate

Primary Target: rat brain PKC
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
125313-65-7C₂₄H₂₂N₄O₂ · C₂H₄O₂ • H2O

Products

Catalogue NumberPackaging Qty/Pack
557508-1MGCN 塑膠安瓿;塑膠針藥瓶 1 mg
Description
OverviewA cell permeable, reversible, selective protein kinase C (PKC) inhibitor (IC50 = 158 nM for rat brain PKC) that acts at the ATP binding site of PKC. Does not inhibit the tyrosine phosphorylation or the activation of phospholipase Cγ1. Exhibits some degree of PKC isozyme specificity (IC50 = 53 nM for PKCα, 195 nM for PKCβI, 163 nM for PKCβII, 213 nM for PKCγ, and 175 nM for PKCε). Inhibits carbachol-evoked noradrenaline release (IC50 = 600 nM).
Catalogue Number557508
Brand Family Calbiochem®
Synonyms2-[1-3(Aminopropyl)indol-3-yl]-3(1-methyl-1H-indol-3-yl)maleimide, Acetate, Bisindolylmaleimide VIII, Acetate
References
ReferencesZhang, C., et al. 1997. J. Immunol. 158, 4968.
Turner, N.A., et al. 1996. J. Neurochem. 66, 2381.
Ozawa, K., et al. 1993. J. Biol. Chem. 268, 1749.
Wilkinson, S.E., et al. 1993. Biochem. J. 294, 335.
Product Information
CAS number125313-65-7
ATP CompetitiveY
FormRed to reddish-orange solid
Hill FormulaC₂₄H₂₂N₄O₂ · C₂H₄O₂ • H2O
Chemical formulaC₂₄H₂₂N₄O₂ · C₂H₄O₂ • H2O
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetrat brain PKC
Primary Target IC<sub>50</sub>158 nM against rat brain PKC; 53 nM for PKCα, 195 nM for PKCβ, 163 nM for PKCβII, 213 nM for PKCγ, and 175 nM for PKCε
Purity≥93% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
557508-1MGCN 04055977268126

Documentation

Ro-31-7549, Monohydrate - CAS 125313-65-7 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Ro-31-7549, Monohydrate - CAS 125313-65-7 - Calbiochem Certificates of Analysis

TitleLot Number
557508

References

Reference overview
Zhang, C., et al. 1997. J. Immunol. 158, 4968.
Turner, N.A., et al. 1996. J. Neurochem. 66, 2381.
Ozawa, K., et al. 1993. J. Biol. Chem. 268, 1749.
Wilkinson, S.E., et al. 1993. Biochem. J. 294, 335.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-January-2010 RFH
Synonyms2-[1-3(Aminopropyl)indol-3-yl]-3(1-methyl-1H-indol-3-yl)maleimide, Acetate, Bisindolylmaleimide VIII, Acetate
DescriptionA cell-permeable, reversible, selective protein kinase C (PKC) inhibitor that acts at the ATP binding site of PKC (IC50 = 158 nM for rat brain PKC). Does not inhibit the tyrosine phosphorylation or the activation of phospholipase Cγ1 unlike staurosporine and other serine/threonine kinase inhibitors. IC50 values for individual PKC isozymes are as follows: 53 nM for PKCα, 195 nM for PKCβI, 163 nM for PKCβII, 213 nM for PKCγ, and 175 nM for PKCε. Inhibits carbachol-evoked noradrenaline release (IC50 = 0.6 µM).
FormRed to reddish-orange solid
CAS number125313-65-7
Chemical formulaC₂₄H₂₂N₄O₂ · C₂H₄O₂ • H2O
Structure formulaStructure formula
Purity≥93% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesZhang, C., et al. 1997. J. Immunol. 158, 4968.
Turner, N.A., et al. 1996. J. Neurochem. 66, 2381.
Ozawa, K., et al. 1993. J. Biol. Chem. 268, 1749.
Wilkinson, S.E., et al. 1993. Biochem. J. 294, 335.