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ECM512 QCM Chemotaxis Cell Migration Assay, 96-well (5 µm), fluorimetric

The QCM 5 um 96-well Migration Assay utilizes a 5 um pore size, which is appropriate for studying monocyte/macrophage migration.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Activity Assay
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ECM512
1 plate  96 wells
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Overview

Replacement Information

Key Spec Table

Key ApplicationsDetection Methods
ACTFluorescent
Description
Catalogue NumberECM512
Brand Family Chemicon®
Trade Name
  • QCM
  • Chemicon
DescriptionQCM Chemotaxis Cell Migration Assay, 96-well (5 µm), fluorimetric
OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (Clark et al., 1996; Martin, 1997). In immune responses, monocyte migration is critical for providing immunological defense and eliminating infectious agents. Monocytes (macrophage precursors) circulate the blood until encountering specific chemotactic factors that initiate the migration process. Once engaged, monocytic cells transverse the vascular endothelium in a multistep mechanism by: A) E-selectin mediated monocyte rolling B) cell adhesion C) transmigration into the subendothelium (Ashida et al., 2001). During this migration and entering the tissue, monocytes develop/differentiate into mature macrophages, increasing in size, phagocytic activity, and proteolytic enzyme secretion. Once at the site of infection, mature macrophage respond to infection by phagocytosis, cytokine secretion, respiratory burst, T-cell antigen presentation, and digestive enzyme secretion (Holevinsky & Nelson, 1995).

Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the topside of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.

The Chemicon QCM™ 96-well 5 μm Migration Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples (Gildea et al., 2000).

In the Chemicon QCM™ 5 μm 96-well Migration Assay, migratory cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQuant GR® dye (Molecular Probes). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (Jones et al., 2001).

Most migration assays utilize an 8 μm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Chemicon QCM™ 5 μm 96-well Migration Assay utilizes a 5 μm pore size, which is appropriate for studying monocyte/macrophage migration. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.

The Chemicon QCM™ 5 μm 96-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.

In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

  • QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
  • QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
  • QCM™ 96-well Cell Invasion Assay (ECM555)
  • 24-well Insert Cell Migration and Invasion Assay Systems
  • CytoMatrix™ Cell Adhesion strips (ECM protein coated)
  • QuantiMatrix™ ECM protein ELISA kits

    Application

    The Chemicon QCM™ 96-well 5μm Migration Assay is ideal for studying chemotaxis cell migration. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents. The 5 μm pore size of this assay's Boyden chambers is appropriate for studying monocyte/ macrophage migration. Each kit provides sufficient materials for the evaluation of 96 samples.

    The Chemicon QCM™ 5μm 96-well Migration Assay is intended for research use only; not for diagnostic applications.
    		•
    References
    Product Information
    Detection methodFluorescent
    Quality LevelMQ100
    Applications
    ApplicationThe QCM 5 um 96-well Migration Assay utilizes a 5 um pore size, which is appropriate for studying monocyte/macrophage migration.
    Key Applications
    • Activity Assay
    Biological Information
    Species Reactivity
    • All
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Usage Statement
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Storage and Shipping Information
    Packaging Information
    Material Size1 plate
    Material Package96 wells
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    ECM512 04053252278402

    Documentation

    QCM Chemotaxis Cell Migration Assay, 96-well (5 µm), fluorimetric MSDS

    Title

    Safety Data Sheet (SDS) 

    References

    Reference overviewPub Med ID
    Toxicity and intraocular properties of a novel long-acting anti-proliferative and anti-angiogenic compound IMS2186.
    Iryna A Falkenstein,Lingyun Cheng,Flossie Wong-Staal,Ajay M Tammewar,Erin C Barron,Gabriel A Silva,Qi-Xiang Li,Dehua Yu,Michelle Hysell,Guohong Liu,Ning Ke,James E Macdonald,William R Freeman
    Current eye research  33  2008

    Show Abstract Full Text Article    		18600493 18600493

    Brochure

    Title
    Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
    Cell Migration and Invasion: Choosing the Right Assay
    EpiGRO™, EndoGRO™ and FibroGRO™ Reagents

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    Categories

    Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays