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ECM201 Sigma-AldrichQCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric

This QCM 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric is ideal for the study of endothelial cell migration in response to an angiogenic stimulus.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
References
Brochures

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Overview

Replacement Information

Key Spec Table

Detection Methods
Colorimetric Fluorescent

Description
Catalogue Number	ECM201
Trade Name	QCM Chemicon
Description	QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric
Overview	Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here Introduction Angiogenesis is a fundamental process involving the growth of new blood vessels from pre-existing vessels. It is important in development and wound healing, as well as pathologic diseases such as diabetic retinopathy and cancer. During angiogenesis, endothelial cells need to move out of existing vessels, migrate into new areas, proliferate and assemble into new capillaries. The migration of endothelial cells is regulated by many angiogenic factors and anti-angiogenic factors. It is critical for researchers to understand the mechanisms of endothelial cell migration. Millipore's 3 μm QCM™ Endothelial Cell Migration Assay – Fibronectin, Fluorometric provides a quick and efficient system to study the ability of compounds to induce or inhibit endothelial cell migration. This assay also allows screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for endothelial cell migration, analysis of gene function in transfected cells, and determination of ECM protein involvement in cell movement. This versatile assay permits counting of individual migratory cells, and, more importantly, allows quantitative analysis by optical density (OD) using a standard microplate reader. This convenient assay allows large scale screening and quantitative comparison of multiple samples and includes individual migration controls for each sample.
Materials Required but Not Delivered	1. Endothelial cells (for example: HUVEC cells), and cell culture medium (for example: EGM-2). 2. Serum-free medium, such as serum-free EGM-2 containing 0.5% BSA. 3. Sterile PBS or HBSS to wash cells. 4. Distilled water 5. (Optional) Chemoattractant or pharmacological agent for addition to culture medium. 6. Low speed centrifuge and tubes for cell harvesting 7. CO₂ incubator appropriate for subject cells 8. Hemocytometer or other means of counting cells. 9. Trypan blue or equivalent viability stain 10. Fluorescence plate reader.

References

Product Information
Components	1. Fibronectin Test Plate: One 24-well culture plate, containing 12 human FN-coated Boyden chambers, sufficient for the evaluation of 12 test samples. 2. BSA Control Plate: One 24-well culture plate, containing 12 BSA-coated Boyden chambers, sufficient for the evaluation of 12 controls. 3. 4X Cell Lysis Buffer - One 16 mL bottle 4. CyQuant GR Dye®: One vial 75 μL 5. 24-Well Cell Culture Tray 6. 96-Well Stain Quantitation Plate 7. Forceps: 1 pair 8. Accutase (cell detachment solution): One 100 mL bottle
Detection method	Colorimetric Fluorescent
Quality Level	MQ100

Applications
Application	This QCM 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric is ideal for the study of endothelial cell migration in response to an angiogenic stimulus.
Application Notes	The Millipore QCM™ 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric is ideal for the study of endothelial cell migration in response to an angiogenic stimulus. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 3 μm pore size in this assay is optimal for endothelial cells such as HUVEC, but not sufficient for fibroblast migration. The Millipore QCM™ 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric assay is intended for research use only; not for diagnostic applications. Each kit provides sufficient materials for the evaluation of 12 samples.

Applications

Application

This QCM 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric is ideal for the study of endothelial cell migration in response to an angiogenic stimulus.

Application Notes

The Millipore QCM™ 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric is ideal for the study of endothelial cell migration in response to an angiogenic stimulus. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 3 μm pore size in this assay is optimal for endothelial cells such as HUVEC, but not sufficient for fibroblast migration. The Millipore QCM™ 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric assay is intended for research use only; not for diagnostic applications.
Each kit provides sufficient materials for the evaluation of 12 samples.

Biological Information
Species Reactivity	Vertebrates

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	• The ECM201-1 kit components should be stored at 2° to 8°C up until the expiration date provided on the labels. DO NOT FREEZE. • The accompanying 100ml bottle of Accutase also included in this kit should be stored at -20°C.

Packaging Information
Material Size	1 kit
Material Package	Sufficient for 12 assays

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
ECM201	04053252001048

Documentation

QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric MSDS

Title
Safety Data Sheet (SDS)

References

Reference overview	Pub Med ID
Effects of lycopene and apigenin on human umbilical vein endothelial cells in vitro under angiogenic stimulation. Mehmet Sahin,Emel Sahin,Saadet Gümüşlü Acta histochemica 114 2012 Show Abstract Angiogenesis is the formation process of new blood vessels from preexisting vessels. Solid tumors need angiogenesis for growth and metastasis. The suppression of tumor growth by inhibition of neoangiogenic processes represents a potential approach to cancer treatment. Lycopene has powerful antioxidant capacities and anticarcinogenic properties. The aim of this study was to investigate the effects of lycopene on angiogenesis in vitro. For this reason, we measured in vitro angiogenesis in human umbilical vein endothelial cells including parameters of cell proliferation, tube formation, cell migration. Lycopene and apigenin were observed to block the endothelial cell proliferation in a dose-dependent manner. In addition, they significantly decreased the capillary-like tube lengths, tube formation and endothelial cell migration. This study provides indications that apigenin and lycopene, which are considered as chemopreventive agents, to be effective in vitro on endothelial cells and angiogenesis.	21474164

Reference overview

Pub Med ID

Brochure

Title
Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
Cell Migration and Invasion: Choosing the Right Assay

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ECM201 Sigma-AldrichQCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric

This QCM 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric is ideal for the study of endothelial cell migration in response to an angiogenic stimulus.

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