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48-611MAG MILLIPLEX Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Signaling
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48-611MAG
96-well plate  96-well plate
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Akt (Ser473) GSK3α (Ser21) GSK3β (Ser9) IGF1R (Tyr1135/Tyr1136) IR (Tyr1162/Tyr1163) IRS1 (Ser636) mTOR (Ser2448) p70S6K (Thr412) PTEN (Ser380) RPS6 (Ser235/Ser236) TSC2 (Ser939) HMplexLuminex xMAP
Description
Catalogue Number48-611MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay
OverviewThe Akt signaling pathway, one of the most often dysregulated signaling pathways in cancer, plays an important role in mediating a very broad range of cellular processes such as growth and development, cell cycle, energy homeostasis, and cell survival. Akt, a serine/threonine kinase that phosphorylates over 100 protein substrates, mediating cell survival and proliferation signals, is often itself hyperphosphorylated in various tumor types. Although the Akt gene itself is not known to be mutated in cancers, many of the upstream regulators of Akt, including IR, IRS, PI3K and PTEN, are oncogenes and tumor suppressors. Downstream of Akt, the mammalian Target Of Rapamycin (mTOR) complex is a key regulator of growth and metabolism. As nearly all of the players in the Akt/mTOR signaling pathway are coordinately regulated by phosphorylation, understanding the role of this pathway in normal physiological processes and in diseases such as cancer and diabetes requires the ability to simultaneously measure phosphorylation status of multiple protein targets.
References
Product Information
Detection methodLuminex xMAP
HS Code3002 15 90
ConfigurationPremixed
Precision, %
  • Intra-Assay: < 7
  • Inter-Assay: < 20
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Akt (Ser473)
  • GSK3α (Ser21)
  • GSK3β (Ser9)
  • IGF1R (Tyr1135/Tyr1136)
  • IR (Tyr1162/Tyr1163)
  • IRS1 (Ser636)
  • mTOR (Ser2448)
  • p70S6K (Thr412)
  • PTEN (Ser380)
  • RPS6 (Ser235/Ser236)
  • TSC2 (Ser939)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
48-611MAG 04053252757501

Documentation

Protocols

Title
Protocol- 48-611MAG

MILLIPLEX Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay MSDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single

Posters

Title
Biological Evaluation of Human PBMC and Th17 cells using MILLIPLEX®MAP Cell Signaling and Cytokine Panels