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CBA001 InnoZyme™ Cathepsin B Activity Assay Kit, Fluorogenic

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CBA001
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Overview

Key Spec Table

Detection Methods
Fluorometric

Products

Catalogue NumberPackaging Qty/Pack
CBA001-1KITCN 玻璃瓶 1 kit
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Description
OverviewA sensitive fluorogenic assay for the measurement of cathepsin B activity (Excitation max: ~360-380 nm; Emission max: ~430-460 nm). Cathepsin B is a cysteine proteinase which has been associated with increased invasivessness and development of the malignant cell phenotype. Cathepsin B has also been implicated in inflammatory airway disease.
Catalogue NumberCBA001
Brand Family Calbiochem®
Application Data
Calibration curve generated by various dilutions of the free AMC standard included in the kit (above).
Materials Required but Not Delivered• Pipettors or multi-channel pipettors calibrated to target volume
• 37°C incubator
• Microplate reader capable of measuring fluorescence at wavelengths 360-380 nm for excitation and 440-460 nm for emission
• Ice bucket
References
ReferencesBervar, A., et al. 2003. Biol. Chem. 384, 447.
Grigolo, B. et al. 2003. Biomaterials 24, 1751.
Scolaris, A., et al. 2002. Biol. Chem. 383, 1297.
Murata, M., et al. 1991. FEBS Lett. 280, 307.
Berquin, I.M. and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.
Burnett, D., 1995. Arch. Biochem. Biophys. 317, 305.
Reddy, V.Y., et al. 1995. Proc. Natl. Acad. Sci. 92, 3849.
Buttle, D.J., 1994. In: Immunopharmacology of Joints and Connective Tissue (Dingle, J.T. and Davies, M.E., eds) London: Academic Press. 225.
Barrett, A.J. and Kirschke, H., 1981. Methods Enzymol. 80, 535.
Product Information
Detection methodFluorometric
Form96 Tests
Format96-well plate
Kit containsCathepsin B Enzyme, Calibration Standard, Cathepsin B Substrate, Assay Buffer, Cathepsin B Inhibitor, Reduction Reagent, Cell Lysis Buffer, Microtiter Plate, Plate Sealer, and a user protocol.
Positive controlCathepsin B
Quality LevelMQ100
Biological Information
Assay range0.09 - 100 ng/ml
Assay time30 min
Sample TypeCells and tissues
Physicochemical Information
Sensitivity0.63 ng/ml
Product Usage Statements
Intended useThe Calbiochem® Cathepsin B Activity Assay Kit, Fluorogenic is designed to measure cathepsin B activity in tissues extracts and cell lysates, and for screening cathepsin B inhibitors.

Note: Following initial use the control Cathepsin B should be dispensed into aliquots and stored at -20°C. Avoid freeze/thaw cycles
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Storage ConditionsStore the unopened components at -20°C and -70°C as indicated on the labels. All kit components, once opened, can be stored under the following conditions:

Assay Buffer, Cell Lysis Buffer, 96-Well Plate, and Plate Sealers at Room Temperature

Cathepsin B Inhibitor, Reduction Reagent, Cathepsin B Substrate, and Calibration Standard at -20°C

Control Cathepsin B(aliquot) at -70°C
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Supplemental Information
Kit containsCathepsin B Enzyme, Calibration Standard, Cathepsin B Substrate, Assay Buffer, Cathepsin B Inhibitor, Reduction Reagent, Cell Lysis Buffer, Microtiter Plate, Plate Sealer, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
CBA001-1KITCN 04055977220810