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IPVH304F0 Immobilon®-P PVDF Membrane

10 sheets, 26 cm x 26 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Immobilon®-P PVDF Membrane MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Dot Blot
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IPVH304F0
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Overview

Replacement Information

Key Spec Table

Key Applications
WB, DB
Description
Catalogue NumberIPVH304F0
Trade Name
  • Immobilon
DescriptionImmobilon®-P PVDF Membrane
Background InformationOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References
Product Information
Detection methodChemiluminescence Chromogenic Radioactive
Compatible Stains
  • Coomassie brilliant blue
  • Amido black
  • India ink
  • Ponceau-S red
  • Colloidal gold
  • CPTS
  • Toluidine blue
  • Transillumination
  • Sypro® ruby
Filter CodeIPVH
Filter ColorWhite
Quality LevelMQ400
Applications
Application10 sheets, 26 cm x 26 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
Key Applications
  • Western Blotting
  • Dot Blot
Biological Information
Primary Binding MechanismsElectrostatic, hydrophobic
BSA Adsorption215 µg/cm²
Goat IgG Adsorption294 µg/cm²
Insulin Adsorption160 µg/cm²
WettabilityHydrophobic
Physicochemical Information
Pore Size0.45 µm
Dimensions
Filter SurfacePlain
Filter Dimensions26 cm x 26 cm
Materials Information
Chemistry
  • Hydrophobic Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size10
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IPVH304F0 04053252471339

Documentation

Immobilon®-P PVDF Membrane MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewApplication
Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
Luo S., Wehr N.B., Levine R.L.
Analytical Biochemistry:350 (2006):233-238  2006

Immunoblotting (Western)

Brochure

Title
Western Blotting Tools

Technical Info

Title
Protein Blotting Handbook: 6th Edition

Data Sheet

Title
Immobilon Transfer Membranes: For superior protein and nucleic acid blots

User Guides

Title
Immobilon -P Blotting Sandwiches
Immobilon®-P Transfer Membrane User Guide