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SCR600 Human Oligodendrocyte Differentiation Kit

The Human Oligodendrocyte Differentiation Kit contains Human Oligodendrocyte Progenitor Cells (OPCs) along with optimized media for their expansion & spontaneous differentiation into mature oligodendrocytes.

Human Oligodendrocyte Differentiation Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
SCR600
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCR600
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionHuman Oligodendrocyte Differentiation Kit
OverviewEMD Millipore’s Human Oligodendrocyte Differentiation Kit contains Human Oligodendrocyte Progenitor Cells (OPCs) along with optimized media for their expansion and spontaneous differentiation into mature oligodendrocytes. Human Oligodendrocyte Progenitor Cells (OPCs) are derived from NIH approved H9 human embryonic stem cells (hESCs). These hESC-derived OPCs proliferate as an adherent cell monolayer and greater than 70% express the appropriate early-to-intermediate oligodendrocyte markers, including O4, NG2, Olig2, Sox10 and GalC. Upon growth factor withdrawal, Human OPCs spontaneously differentiate into mature oligodendrocytes expressing terminally differentiated markers including proteolipid protein (PLP), myelin basic protein (MBP), and myelin oligodendrocyte glycoprotein (MOG). Spontaneous differentiation leads to approximately 30% of the cells expressing PLP, MBP and MOG while the remaining 70% express mostly neuronal markers, MAP2 and beta III-tubulin with very little to no astrocytes (<5% GFAP-positive cells) observed. Human OPCs can be used for a variety of research applications including studies of neurotoxicity, co-culture applications and screening for molecules that induce or inhibit preferential differentiation to mature oligodendrocytes.
References
Product Information
Components
  • Human Oligodendrocyte Progenitors (OPCs), 500,000 viable cells: (Cat. No. CS204496)
  • Human OPC Expansion Media Kit, 100 mLs: (Cat. No. SCM107)
  • Human OPC Spontaneous Differentiation Media Kit, 100 mLs: (Cat. No. SCM106)
Quality LevelMQ100
Applications
ApplicationThe Human Oligodendrocyte Differentiation Kit contains Human Oligodendrocyte Progenitor Cells (OPCs) along with optimized media for their expansion & spontaneous differentiation into mature oligodendrocytes.
Key Applications
  • Stem Cell Culture
Biological Information
Stem Cell Type
  • Neural Stem Cells
Cell Line Type
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceSterility testing: Negative for bacterial and fungal growth
pH: 7.4 +/- 0.4
Osmolality: 290 +/- 20mOsm
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsPlease see manual for recommended storage conditions for individual components
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR600 04053252017261

Documentation

Brochure

Title
Human Stem Cell Systems
Neuroscience Product Selection Guide
Simplifying the iPSC Workflow

Technical Info

Title
Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

Data Sheet

Title
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

Posters

Title
A unifying neural differentiation platform to direct lineage-specific neurons and glial cells from human pluripotent stem cells

User Guides

Title
Human Oligodendrocyte Differentiation Kit

Newsletters / Publications

Title
Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011