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03-226 HDAC6 (His-tag), human recombinant

Human recombinant produced in insect cells that can be used to deacetylate various acetylated substrates.

HDAC6 (His-tag), human recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
03-226
50 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpecies
Enzyme AssaysHuman
Description
Catalogue Number03-226
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHDAC6 (His-tag), human recombinant
OverviewHuman recombinant HDAC6 (His-tag) produced in insect cells.
Background InformationHistone deacetylases (HDACs) are chromatin-remodeling factors that deacetylate histones and act as transcriptional repressors or silencers of genes. They regulate histone acetylation by catalyzing the removal of acetyl groups on the amino terminal lysine residues of the core nucleosomal histones. Each cell is reported to have several different types of HDACs. HDAC6 is a member of the Class II HDACs that displays limited tissue distribution and can shuttle between nucleus and cytoplasm. In the cytoplasm, it is it is reported to associate with microtubules. HDAC6 contains two leucine-rich nuclear export sequences (NES1, NES2) that play an essential role in the cytoplasmic/nuclear shuttling process. The amino acid residues in HDAC6 are arranged in the space to form two independent catalytic domains with a zinc finger ubiquitin-binding domain located at the C-terminus. HDAC6 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4).
References
Product Information
FormatPurified
PresentationIn liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant produced in insect cells that can be used to deacetylate various acetylated substrates.
Key Applications
  • Enzyme Assays
Application NotesHDAC deacetylase assays
Biological Information
PurityPurity >90% by SDS-PAGE
SourceHuman HDAC6
Specific Activity>2 U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
Molecular Weight~131 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC6 into separate tubes before storing at -80°C.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-226 04053252550874

Documentation

HDAC6 (His-tag), human recombinant MSDS

Title

Safety Data Sheet (SDS) 

HDAC6 (His-tag), human recombinant Certificates of Analysis

TitleLot Number
HDAC6 (His-tag) human recombinant - 2394041 2394041
HDAC6 (His-tag) human recombinant - 3250215 3250215
HDAC6 (His-tag) human recombinant - 3422207 3422207
HDAC6 (His-tag) human recombinant - 3870783 3870783
HDAC6 (His-tag) human recombinant - 4086535 4086535
HDAC6 (His-tag) human recombinant - 4180221 4180221
HDAC6 (His-tag) human recombinant - Q2022479 Q2022479
HDAC6 (His-tag) human recombinant -2652555 2652555
HDAC6 (His-tag) human recombinant Recombinant Protein 2926420

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