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SCC284 EpH4 Mouse Mammary Epithelial Cell Line

EpH4 mouse mammary epithelial cell line is a versatile system for studying a spectrum of morphological transformation spanning breast cancer metastasis, EMT, and hormonal regulation.

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SCC284
≥1X10⁶ cells/vial   
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Overview

Replacement Information
Description
Catalogue NumberSCC284
DescriptionEpH4 Mouse Mammary Epithelial Cell Line
Alternate Names
  • EPH4
  • EpH-4
  • Eph4
Background InformationMammary epithelial cells are a model for cellular plasticity, the ability to undergo varied morphological changes. One of the significant aspects of mammary-derived tumors is epithelial-to-mesenchymal transition (EMT), which gives rise to the migratory behavior required for metastasis (1). Cellular plasticity makes mammary epithelial cell models valuable for the study of breast cancer malignancy in addition to hormonal regulation of differentiation and epithelial cell polarization.

Eph4 is a spontaneously immortalized cell line derived from primary murine mammary epithelial cells (2). EpH4 cells can be induced to synthesize milk proteins, such as caseins, upon the induction with lactogenic hormones such as prolactin, hydrocortisone, and insulin. For normal maintenance Eph4 cells should be cultured at high density (semi-confluency) and passaged before reaching confluency to avoid polarization and selective culture of single cells. Post-confluent Eph4 cells undergo polarization and formation of hemicysts or dome-like structures characterized by unidirectional transport of ions (3).

Source:
Eph4 cell line is a spontaneously immortalized clone derived from primary mammary epithelial cells of a female BALB/c mouse.

References:
1. Breast Cancer Res. 2005; 7(4): 171-179.
2. EMBO J 1995; 14(6): 1145-55.
3. J Cell Biol. 1989; 108(3): 1127-38.
References
Product Information
Applications
ApplicationEpH4 mouse mammary epithelial cell line is a versatile system for studying a spectrum of morphological transformation spanning breast cancer metastasis, EMT, and hormonal regulation.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Epithelial Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC284 04061842104017