Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

71745 D-Tube™ Dialyzer Mega, 20 ml, MWCO 3.5 kDa

D-Tube™ Dialyzer Mega, 20 ml, MWCO 3.5 kDa MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71745
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71745-3CN 玻璃瓶 10 d-tu
71745-4CN 玻璃瓶 50 d-tu
Description
Overview
D-Tube™ Dialyzers Mini, Midi, Maxi, and Mega
The D-Tube™ Dialyzers be used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuge, or laborious steps to manipulate small sample volumes. The sample is added and removed using a standard laboratory pipet. Available with molecular weight cutoffs (MWCO) from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in four volume capacities: mini (10-250 µl), midi (50-800 micro;l), maxi (500-3000 µl), and mega (10ml, 15ml, and 20 ml). The membrane is ultra-clean, EDTA-treated regenerated cellulose, sulfur- and heavy metal-free.

The D-Tube Dialyzers Mini, Midi, and Maxi contain 10 D-Tube Dialyzers and one floating rack that can hold up to four D-Tube Dialyzers. The D-Tube Dialyzers Mega contain 10 or 50 tubes and one or 5 floating racks to hold one D-Tube Mega in the exchange buffer.

D-Tube™ Electroelution Accessory Kit
The D-Tube Electroelution Accessory Kit provides a support tray for the D-Tube Dialyzers (compatible with most commercially-available horizontal electrophoresis units) and optimized reagents for protein and nucleic acid precipitation following electroelution. The kit also contains an optimized buffer for the removal of SDS from extracted protein samples, making them compatible with MALDI-MS analysis. The combination of D-Tube Dialyzers and D-Tube Electroelution Accessory Kit provides a unique tool for extraction of any protein, protein-protein and protein-DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels with 60% recovery yield in less than 2 hours. The procedure efficiently recovers proteins and simultaneously removes ampholytes from proteins run on 2D gels. Extracted proteins are compatible with most downstream applications such as MALDIMS, animal immunization for antibody production, HPLC analysis, peptide mapping, and functional assays. In addition, D-Tube Dialyzers can be used for oligonucleotides, RNA and DNA extraction from both polyacrylamide and agarose gels. Efficient extraction (>90%) is achieved for 15 nt oligos and for up to 80 kbp DNA fragments.
Catalogue Number71745
Brand Family Novagen®
Features and benefits
  • Easy-to-handle dialyzers for buffer exchange and removal of urea, detergents
  • Ideal for electroelution of proteins, protein-DNA complexes, oligonucleotides, DNA, and RNA from polyacrylamide and agarose gels
  • One-step procedure that does not require syringes or any special equipment
  • Sample volume recovery > 97%
  • Protease, RNAse, DNAse, and PCR product free
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71745-3CN 04055977266955
71745-4CN 04055977266962

Related Products & Applications

Related Products

Catalogue Number Description
71739 D-Tube™ Dialyzer Mega, 10 ml, MWCO 3.5 kDa
71740 D-Tube™ Dialyzer Mega, 10 ml, MWCO 6-8 kDa
71742 D-Tube™ Dialyzer Mega, 15 ml, MWCO 3.5 kDa
71743 D-Tube™ Dialyzer Mega, 15 ml, MWCO 6-8 kDa
71746 D-Tube™ Dialyzer Mega, 20 ml, MWCO 6-8 kDa
71748 Floating Rack, Mega

Related Products By: Brand Facete

D-Tube™

Categories

Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Protein Dialyzers