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SCR103 Collagenase type I

The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

Collagenase type I MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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SCR103
250 mg  1 vial containing 250mg of lyophilized powder
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Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCR103
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionCollagenase type I
OverviewCollagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.
Materials Required but Not Delivered• Ultrapure water (e.g., MilliQ)
• 0.22 μM sterile filtration device (e.g. Millipore’s Stericup-GP, Catalog # SCGPU01RE)
References
Product Information
HS Code3507 90 19
Formulation
Quality LevelMQ100
Applications
ApplicationThe collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
Key Applications
  • Stem Cell Culture
Biological Information
Media FormLiquid
Stem Cell Type
  • Human Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEach lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore's HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsCollagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.
Packaging Information
Material Size250 mg
Material Package1 vial containing 250mg of lyophilized powder
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR103 04053252397868

Documentation

Collagenase type I MSDS

Title

Safety Data Sheet (SDS) 

Collagenase type I Certificates of Analysis

TitleLot Number
Collagenase type I 3079679
Collagenase type I 2978576
Collagenase type I 3034146
Collagenase type I 3130613
Collagenase type I 3022083
Collagenase type I 3049470
Collagenase type I 2909011
Collagenase type I 3003794
Collagenase type I 3099012
Collagenase type I -
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Technical Info

Title
Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium