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17-10199 Sigma-AldrichChIPAb+™ HDAC1 Antibody, rabbit polyclonal

This ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

ChIPAb+™ HDAC1 Antibody, rabbit polyclonal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Key Applications: Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones

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Key Spec Table

Species Reactivity	Key Applications
M, H, R, Ca, Ht	WB, ICC, ChIP

Description
Catalogue Number	17-10199
Trade Name	ChIPAb+ Upstate
Description	ChIPAb+™ HDAC1 Antibody, rabbit polyclonal
Overview	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ HDAC1 polyclonal set includes the HDAC1 antibody, a Normal Rabbit IgG, and control primers which amplify a 88 bp region of ChIP Primers p21. The HDAC1 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1-associated chromatin.

References

Product Information
Format	Purified
Control	Includes normal rabbit IgG and primers specific for human p21.
Presentation	Anti-HDAC1 (rabbit polyclonal). One vial containing 50 µg of Anti-HDAC1 IgG in PBS with 1 mg/mL BSA and ≤0.1% sodium azide before the addition of glycerol to 30%. Store at -20°C. Concentration: 0.7 mg/mL Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C. FOR: CCC ACA GCA GAG GAG AAA GAA REV: CTG GAA ATC TCT GCC CAG ACA
Quality Level	MQ100

Applications
Application	This ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	Western Blotting Immunocytochemistry Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 . (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. 0.5 µg/mL from a representative lot detected HDAC1 in HeLa cells. (Figure 3). Immunofluorescence Analysis: 2 µg/mL from a representative lot detected HDAC1 in HeLa cells.

Biological Information
Immunogen	Synthetic peptide corresponding to amino acids 467-482 of human HDAC1.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Species Reactivity	Mouse Human Rat Canine Hamster
Species Reactivity Note	Demonstrated to react with human. Predicted to react with rat, mouse, canine, and hamster based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_032254
Molecular Weight	~62 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 flanking an Sp1 binding site in the human p21 promoter (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 flanking an Sp1 binding site in the human p21 promoter (Figure 1).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	25 assays
Material Package	25 assays per set. Recommended use: ~2 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
17-10199	04053252296628

Documentation

ChIPAb+™ HDAC1 Antibody, rabbit polyclonal MSDS

Title
Safety Data Sheet (SDS)

ChIPAb+™ HDAC1 Antibody, rabbit polyclonal Certificates of Analysis

Title	Lot Number
ChIPAb+ HDAC1 - 2053033	2053033
ChIPAb+ HDAC1 - Q1922566	Q1922566
ChIPAb+ HDAC1 - 2561140	2561140
ChIPAb+ HDAC1 - 3383260	3383260
ChIPAb+ HDAC1 - 3598089	3598089
ChIPAb+ HDAC1 - 3698772	3698772
ChIPAb+ HDAC1 - 3924105	3924105
ChIPAb+ HDAC1 - 4155314	4155314
ChIPAb+ HDAC1 -2584062	2584062
ChIPAb+ HDAC1 -2611211	2611211

Brochure

Title
Advance your Epigenetics Research
New Products: Volume 3, 2012

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