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235423 Caspase-3 Inhibitor I, Cell-Permeable - Calbiochem

The Caspase-3 Inhibitor I, Cell-Permeable controls the biological activity of Caspase-3. This small molecule/inhibitor is primarily used for Cancer applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO

Primary Target: caspase-3
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235423
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₉₄H₁₅₈N₂₀O₂₇

Products

Catalogue NumberPackaging Qty/Pack
235423-1MGCN 塑膠安瓿;塑膠針藥瓶 1 mg
Description
OverviewA cell-permeable inhibitor of caspase-3, as well as caspase-6, caspase-7, caspase-8, and caspase-10. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-3 (Ki < 1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC50 = 200 pM). The N-terminal sequence (amino acid residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide. A 5 mM (1 mg/100 µl) solution of Caspase-3 Inhibitor I, Cell-permeable (Cat. No. 235427) in DMSO is also available.
Catalogue Number235423
Brand Family Calbiochem®
SynonymsCPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO
References
ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Lazebnik, Y.A., et al. 1994. Nature 371, 346.
Product Information
ATP CompetitiveN
FormLyophilized solid
Hill FormulaC₉₄H₁₅₈N₂₀O₂₇
Chemical formulaC₉₄H₁₅₈N₂₀O₂₇
ReversibleY
Sold on the basis of peptide contentY
Quality LevelMQ100
Applications
Biological Information
Primary Targetcaspase-3
Primary Target IC<sub>50</sub>200 pM for inhibiting PARP cleavage in cultured human osteosarcoma cell extracts
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Peptide ContentY
Peptide SequenceAc-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
235423-1MGCN 04055977217025

Documentation

Caspase-3 Inhibitor I, Cell-Permeable - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Caspase-3 Inhibitor I, Cell-Permeable - Calbiochem Certificates of Analysis

TitleLot Number
235423

References

Reference overview
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Lazebnik, Y.A., et al. 1994. Nature 371, 346.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Jurg Grunenfelder, et al. (2001) Upregulation of Bcl-2 through caspase-3 inhibition ameliorates ischemia/reperfusion injury in rat cardiac allografts. Circulation 104, I202-I206.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-February-2014 JSW
    SynonymsCPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO
    DescriptionA cell-permeable inhibitor of caspase-3. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent and reversible inhibitor of caspase-3 (Ki <1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC50 = 0.2 nM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.
    FormLyophilized solid
    Chemical formulaC₉₄H₁₅₈N₂₀O₂₇
    Peptide SequenceAc-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO
    Purity≥97% by HPLC
    SolubilityDMSO (5 mg/ml)
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
    Toxicity Standard Handling
    ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
    Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
    Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
    Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
    Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
    Nicholson, D.W., et al. 1995. Nature 376, 37.
    Tewari, M., et al. 1995. Cell 81, 801.
    Lazebnik, Y.A., et al. 1994. Nature 371, 346.
    Citation
  • Jurg Grunenfelder, et al. (2001) Upregulation of Bcl-2 through caspase-3 inhibition ameliorates ischemia/reperfusion injury in rat cardiac allografts. Circulation 104, I202-I206.
    		•