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208771 Calpain Substrate III, Fluorogenic - Calbiochem

A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (DABCYL)-TPLK~SPPPSPR-(EDANS)

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208771
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
208771-1MGCN 塑膠安瓿;塑膠針藥瓶 1 mg
Description
OverviewA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
Catalogue Number208771
Brand Family Calbiochem®
Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
References
ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
Product Information
ATP CompetitiveN
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₈₀H₁₁₄N₂₀O₁₈S
Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetSubstrate sequence that is optimized for calpain-1 and -2
Primary Target IC<sub>50</sub>kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
208771-1MGCN 04055977202854

Documentation

Calpain Substrate III, Fluorogenic - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Calpain Substrate III, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
208771

References

Reference overview
Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-May-2008 RFH
Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
DescriptionA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with higher efficiency (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively). It is kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in intact Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
Purity≥98% by HPLC
SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Irritant
ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.