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QIA58 BrdU Cell Proliferation Assay

This proliferation assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells.

BrdU Cell Proliferation Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Bromodeoxyuridine Assay

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QIA58
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Overview

Key Spec Table

Detection Methods
Colorimetric

Products

Catalogue NumberPackaging Qty/Pack
QIA58-1000TESTCN 纖維紙箱 1000 test
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QIA58-200TESTCN 纖維紙箱 200 test
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Description
OverviewBrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform.
Note: 1 T = 1 test.
Catalogue NumberQIA58
Brand Family Calbiochem®
SynonymsBromodeoxyuridine Assay
Materials Required but Not Delivered• 2-20 µl, 20-200 µl and 200-1000 µl precision pipetters with disposable tips
• Wash bottle or multichannel dispenser for washing
• 500 ml graduated cylinder
• PBS (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4-7H2O, 1.4 mM KH2PO4)
• Deionized or distilled H2O
• Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450-540 or 450-595 nm
• Tissue culture plate (96 well culture dish)
• Reagent trough
• Micro filter (0.2 µm)
• Syringe
References
ReferencesHardonk, M.J. and Harms, G. 1990. Acta histochemica, Suppl. 39, 99.
Muir, D., et al. 1990. Analytical Biochemistry 185, 377.
Lanier, T. L., et al. 1989. Carcinogenesis 10, 1341.
Magaud, J.-P., et al. 1988. J. Immunol. Methods 106, 95.
Collins, S.J. 1987. Blood 70, 1233.
Porstmann, T., et al. 1985. J. Immunol. Methods 82, 169.
Raza, A., et al. 1985. Cytometry 6, 633.
Morstyn, G., et al. 1983. J. Clin. Invest. 72, 1844.
Gratzner, H.G. 1982. Science 218, 474.
Product Information
Detection methodColorimetric
Form200 or 1000 Tests
Format96-well plate
Kit containsBrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol.
Quality LevelMQ100
Biological Information
Assay time3 h
Sample TypeIntact Cells
Safety Information
R PhraseR: 11-20/21/22-35-36/38-46-61

Highly flammable.
Harmful by inhalation, in contact with skin and if swallowed.
Causes severe burns.
Irritating to eyes and skin.
May cause heritable genetic damage.
May cause harm to the unborn child.
S PhraseS: 7-16-26-36/37/39-45

Keep container tightly closed.
Keep away from sources of ignition - No Smoking.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Intended useThe Calbiochem® BrdU Cell Proliferation Assay is a non-isotopic immunoassay for the quantitation of bromodeoxyuridine incorporation into newly synthesized DNA of actively proliferating cells.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Storage ConditionsUpon arrival store the entire contents of the kit at -20°C, in a non-frostfree freezer.

Prior to use:
1. Remove the Fixative/Denaturing Solution and place at room temperature for at least 4 h prior to use. Precipitates that may occur while cold should go back into solution.
2. Reconstitute the Peroxidase Goat Anti-Mouse IgG with the appropriate volume of 1X PBS.
• For the 200 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 125 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
• For the 1000 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 250 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
Allow solution to stand at room temperature for 10 min. (or until solution is clear). Divide into small aliquots; aliquots not to be used that same day should be stored at -20°C in a non-frostfree freezer.

Once the kit components have been thawed for use:
• The BrdU label and 100X Anti-BrdU Antibody should be divided into small aliquots and stored at -20°C in a non-frostfree freezer with the Peroxidase Goat Anti-Mouse IgG; avoid multiple freeze/thaw cycles.
• All the other kit components (Substrate, Antibody Diluent, Conjugate Diluent, 20X Plate Wash Concentrate, Fixative/Denaturing Solution, Stop Solution) should be stored at 4°C.
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Supplemental Information
Kit containsBrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
QIA58-1000TESTCN 04055977209129
QIA58-200TESTCN 04055977209136