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APT115 Apo-BrdU™ Detection Kit

The kit measures apoptosis in cells.

Apo-BrdU™ Detection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Flow Cytometry
APT115
50 assays  
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Key Spec Table

Key ApplicationsDetection Methods
FCFluorescent
Description
Catalogue NumberAPT115
Brand Family Chemicon®
Trade Name
  • Apo-BrdU
  • Chemicon
DescriptionApo-BrdU™ Detection Kit
OverviewThe CHEMICON APO-BRDU™ Kit is a two color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains the instructions and reagents required for measuring apoptosis in cells including; positive and negative control cells for assessing reagent performance; washing, reaction, and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and Propidium Iodide/RNase A solution for counter staining the total DNA.
Materials Required but Not Delivered1. Flow Cytometer

2. Distilled Water

3. 1% (w/v) paraformaldehyde (methanol free) in PBS

4. 70% (v/v) ethanol

5. 37°C Water Bath

6. Ice Bucket

7. 12 x 75 mm flow cytometry test tubes

8. Micro-pipettor
References
Product Information
Components
  • The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures.
  • Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6.
  • Positive Control Cells, Brown cap
  • Negative Control Cells, White cap
  • Wash Buffer, Blue cap
  • Reaction Buffer, Green cap
  • TdT Enzyme, Yellow cap
  • Br-dUTP, Violet cap
  • Rinsing Buffer, Red cap
  • Fluorescein~PRB-1 mAb, Orange cap
  • PI/RNase Staining Buffer, Amber bottle
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationThe kit measures apoptosis in cells.
Key Applications
  • Flow Cytometry
Biological Information
Species Reactivity
  • All
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsPrecautions and Warnings

1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets.

3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
Packaging Information
Material Size50 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
APT115 04053252025198

Documentation

Apo-BrdU™ Detection Kit MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility.
Muhammad Anzar, Liwei He, Mary M Buhr, Thomas G Kroetsch, Karl P Pauls
Biology of reproduction  66  354-60  2002

Show Abstract 		11804948 11804948