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ABN1658 Anti-pro-TRH

Anti-pro-TRH, Cat. No. ABN1658, is a highly specific rabbit polyclonal antibody, that targets pro- Thyrotropin-releasing hormone and has been tested in Immunofluorescence and Western Blotting.

Anti-pro-TRH MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Trh UniProt Number: P01150
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ABN1658
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIF, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberABN1658
DescriptionAnti-pro-TRH
Alternate Names
  • anti-thyrotropin-releasing hormone
  • anti-Ps4
Background InformationPro-thyrotropin-releasing hormone (UniProt: P01150; also known as Pro-TRH, Prothyroliberin, TRH, TSH-releasing factor, Thyrotropin-releasing factor, TRF) is encoded by the Trh gene (Gene ID: 25569) in rat. TRH is a secreted hypothalamic protein that travels across the median eminence to the pituitary via the hypophyseal portal system. It functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. Pro-TRH (TRH-potentiating peptide: Ps4) is co-synthesized with TRH serves as a marker for TRH-containing neurons. Like TRH itself, pro-TRH is biologically active.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-pro-TRH, Cat. No. ABN1658, is a highly specific rabbit polyclonal antibody, that targets pro- Thyrotropin-releasing hormone and has been tested in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected pro-TRH in median eminence of rat and L2 spinal cord tissues. (Courtesy of Dr. Martin Wessendorf at University of Minnesota).

Immunofluorescence Analysis: A representative lot detected pro-TRH in rat brain stem & rat spinal cord tissues. (Wessendorf, M.W., et. al. (1990). J Histochem Cyotchem. 38(12):1859-77).
Biological Information
ImmunogenA linear peptide corresponding to 10 amino acids
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Thyrotropin-releasing hormone-containing neurons in Rat Hypothalamus and spinal cord.
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with mouse due to sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Trh
Purification MethodUnpurified
UniProt Number
Molecular Weight~29 kDa observed; 29.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat hypothalamus tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected pro-TRH in 10 µg of rat hypothalamus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1658 04054839168710