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AB3848-I Anti-phospho Smad1/Smad5/Smad8 (Ser463/465)

Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been tested in Immunohistochemistry and Western Blotting.

Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SMAD1, BSP1, MADH1, MADR1 UniProt Number: Q15797
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AB3848-I
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3848-I
DescriptionAnti-phospho Smad1/Smad5/Smad8 (Ser463/465)
Alternate Names
  • Mothers against decapentaplegic homolog 1
  • BSP-1
  • hSMAD1
  • JV4-1
  • MAD homolog 1
  • Mothers against DPP homolog 1
  • Mad-related protein 1
  • SMAD 1
  • SMAD family member 1
  • Smad1
  • Transforming growth factor-beta-signaling protein 1
Background InformationMothers against decapentaplegic homolog 1 (UniProt Q15797; also known as BSP-1, hSMAD1, JV4-1, MAD homolog 1, Mothers against DPP homolog 1, Mad-related protein 1, SMAD 1, SMAD family member 1, Smad1, Transforming growth factor-beta-signaling protein 1) is encoded by the SMAD1 (also known as BSP1, MADH1, MADR1) gene (Gene ID 4086) in human. Mammalian SMADs constitute a family of transcription factors that are subdivided into three groups. SMAD1, 2, 3, 5, and 9 (a.k.a. SMAD8) are receptor-regulated SMADs or R-SMADs. SMAD4 is the only known mammalian common mediator SMAD or Co-SMAD and it mediates both the TGF- and BMP signalling pathways. SMAD6 and SMAD7 are inhibitory SMADs or I-SMADs that block the activation of R-SMADs by Co-SMAD via competitive binding. SMADs share a similar structure feature consisting of the conserved N-terminal Mad-homology 1 (MH1; a.a. 12-136 of hSMAD1, a.a. 13-137 of hSMAD2, a.a. 16-140 of hSMAD8) and C-terminal MH2 (a.a. 271-465 of hSMAD1 & hSMAD2, a.a. 273-467 of hSMAD8) domains joined by a non-conserved linker region among SMADs. MH1 domain in R-SMADs and Co-SMADs contains N-terminus nuclear localization signals (NLS), while SMAD4 has nuclear export signals (NES) in this domain. The MH2 domain is responsible for gene transactivation as well as for mediating SMAD-receptor, SMAD-SMAD, SMAD-coactivators and SMAD-corepressors interactions. A TGF- ligand (TGF- for SMAD2/3 and BMP for SMAD1/5/8) initiates signalling by binding and engaging type I and type II receptor serine/threonine kinases on the cell surface, allowing T RII-dependent phosphorylation of T RI kinase domain, which then instigates signalling by phosphorylating the last two serine residues within the conserved C-terminal end Ser-Ser-X-Ser (SSXS) motif of SMAD proteins.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibdoy in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been tested in Immunohistochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Smad1/5/8 phosphorylation in mouse & rat embryo tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human SMAD1 C-terminal end sequence phosphorylated on the last two serine residues (S463/S465).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody recognizes SMAD1/5/8 phosphorylated on their last two serine residues within the C-terminal end Ser-Ser-X-Ser (SSXS) motif. Target serine residues are present in human/mouse SMAD1 (S463/S465), rat SMAD1 (S466/S468), human/mouse/rat SMAD5 (S463/S465), human SMAD9 (S465/S467), mouse SMAD9 (S428/S430), and rat SMAD9 (S432/S434), but absent in human SMAD1 spliced isoform 2. SMAD9 is also known as SMAD8. Target bands detection was blocked by the immunogen phosphopeptide, but not by the corresponding non-phosphopeptide.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Mink, Xenopus based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMAD1
  • BSP1
  • MADH1
  • MADR1
Modifications
  • Phosphorylation
  • Phosphorylation
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~55 kDa observed. 52.26/52.16/52.71 kDa (human/mouse/rat SMAD1), 52.26/52.17/52.22 kDa (human/mouse/rat SMAD5), and 52.49/48.64/48.99 kDa. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected an increased SMAD1/5/8 phosphorylation in 10 µg lysate from BMP2-treated HEK293 cells. Target bands detection was blocked by the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB3848-I 04054839034671

Documentation

Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Certificates of Analysis

TitleLot Number
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) 2842871
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 3172384 3172384
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 3258711 3258711
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 3433051 3433051
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 3503842 3503842
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 3670621 3670621
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 3789385 3789385
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 3911630 3911630
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 4051780 4051780
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) - 4091252 4091252
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