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07-892 Sigma-AldrichAnti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody

This Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody is validated for use in WB, IC for the detection of phospho-Pyk2/CAKβ/FAK2 (Tyr402).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTK2B, RAFTK, FAK2, PYK2, FADK2, PTK, CADTK, CAKB, PKB UniProt Number: Q14289

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	07-892
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody
Alternate Names	PTK2B Proline-rich tyrosine kinase 2 Focal adhesion kinase 2 FADK2 Cell adhesion kinase beta RAFTK Calcium-dependent tyrosine kinase CADTK

References

Product Information
Format	Affinity Purified
Presentation	100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Quality Level	MQ100

Applications
Application	This Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody is validated for use in WB, IC for the detection of phospho-Pyk2/CAKβ/FAK2 (Tyr402).
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Pyk2 (Tyr402) in RIPA lysate of Pyk2 transfected CEF cells

Biological Information
Immunogen	Peptide corresponding to amino acid region encompassing the human and rat phospho-Pyk2/CAKβ/FAK2 (Tyr402).
Host	Rabbit
Specificity	Pyk2 phosphorylated on tyrosine residue 402
Isotype	IgG
Species Reactivity	Human Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004103.3 NM_173174.1 NM_173175.1 NM_173176.1
Entrez Gene Summary	This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
Gene Symbol	PTK2B RAFTK FAK2 PYK2 FADK2 PTK CADTK CAKB PKB
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	Q14289
UniProt Summary	FUNCTION: SwissProt: Q14289 # Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. SIZE: 1009 amino acids; 115875 Da SUBUNIT: Interacts with Crk-associated substrate (Cas), PTPNS1 and SH2D3C (By similarity). Interacts with nephrocystin, DDEF2, OPHN1L, SKAP2 and TGFB1I1. SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interaction with nephrocystin induces the membrane-association of the kinase. TISSUE SPECIFICITY: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes. PTM: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha- lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity (By similarity). SIMILARITY: SwissProt: Q14289 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
Molecular Weight	115 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoblot.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
07-892	04053252731129

Documentation

Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody MSDS

Title
Safety Data Sheet (SDS)

Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody Certificates of Analysis

Title	Lot Number
Anti-phospho-Pyk2/CAK#946;/FAK2 (Tyr402) - 2154046	2154046
Anti-phospho-Pyk2/CAKb/FAK2 (Tyr402) - DAM1524064	DAM1524064
Anti-phospho-Pyk2/CAKb/FAK2 (Tyr402) - DAM1601815	DAM1601815
Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) (Affinity purified Rabbit polyclonal IgG) - 2457084	2457084
Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) (Affinity purified Rabbit polyclonal IgG)	3065192
Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) (Affinity purified Rabbit polyclonal IgG) -2505166	2505166
Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) - 3878266	3878266
anti-phospho-Pyk2/CAKbeta;/FAK2 (Tyr402) - 0604027252	0604027252
anti-phospho-Pyk2/CAKbeta;/FAK2 (Tyr402) - DAM1432889	DAM1432889

References

Reference overview	Pub Med ID
Differential signaling by the focal adhesion kinase and cell adhesion kinase beta Schaller, M.D. and Sasaki, T. J. Biol. Chem., 272(40):25319-25325 (1997) 1997	9312150

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