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04-816-I-100UL Anti-phospho-PPARgamma (Ser112) Antibody, clone AW504 rabbit monoclonal

Anti-phospho-PPARgamma (Ser112), clone AW504 rabbit monoclonal, Cat. No. 04-816-I, is a highly specific rabbit monoclonal antibody that targets Peroxisome proliferator-activated receptor gamma and has been tested for use in Western Blotting.

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04-816-I-100UL
100 μL  
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Overview

Replacement Information
Description
Catalogue Number04-816-I-100UL
DescriptionAnti-phospho-PPARgamma (Ser112) Antibody, clone AW504 rabbit monoclonal
Alternate Names
  • Peroxisome proliferator-activated receptor gamma
  • Nuclear receptor subfamily 1 group C member 3
Background InformationPeroxisome proliferator-activated receptor gamma (UniProt: P37231; also known as PPAR-gamma, Nuclear receptor subfamily 1 group C member 3) is encoded by the PPARG (also known as NR1C3) gene (Gene ID: 5468) in human. Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor family of transcription factors that mediate a variety of cellular processes, including glucose and lipid metabolism, inflammatory responses, and regulation of apoptotic cell death. They act by binding to specific peroxisome proliferator-response elements (PPREs) on target genes. Three types of PPARs exist, namely alpha, delta, and gamma. In addition, alternative splicing produces three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind PPREs in the promoter region of target genes. Highest expression of PPAR gamma is found in the adipose tissue and lower levels are detected in skeletal muscle, spleen, heart and liver. It plays a critical role in adipocyte differentiation and fat deposition. PPAR-gamma is phosphorylated in basal conditions and is dephosphorylated upon treatment with ligands. Its dephosphorylation at serine 112 induces its adipogenic activity. PPAR-gamma can be activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE.
References
Product Information
FormatCulture Supernatant
PresentationRabbit monoclonal antibody in cell culture supernatant with 0.05% sodium azide.
Applications
ApplicationAnti-phospho-PPARgamma (Ser112), clone AW504 rabbit monoclonal, Cat. No. 04-816-I, is a highly specific rabbit monoclonal antibody that targets Peroxisome proliferator-activated receptor gamma and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphoserine 112 from human PPAR gamma, isoform 2.
CloneAW504
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityClone AW504 is a rabbit monoclonal antibody that specifically detects PPAR gamma phosphorylated on serine 112.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Canine, Human, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PPARG
  • NR1C3
Modifications
  • Phosphorylation
Purification MethodUnpurified
UniProt Number
Molecular Weight~55 and 58 kDa observed; 57.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3/L1 cells differentiated into adipocyte-like cells.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-PPARgamma (Ser112) in NIH3T3/L1 cells differentiated into adipocyte-like cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-816-I-100UL 04054839961670

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