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04-797 Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39, rabbit monoclonal

Detect phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) using this Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39 validated for use in Mplex & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: MAPK1, p38, ERK, P42MAPK, ERK2, p40, PRKM1, p41mapk, PRKM2, MAPK2, p42-MAPK, p41, ERK-2, ERT1 UniProt Number: P28482
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04-797
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RMplex, WBRbPurifiedMonoclonal Antibody
Description
Catalogue Number04-797
Replaces05-797
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39, rabbit monoclonal
Alternate Names
  • Mitogen-activated protein kinase
  • Extracellular signal-regulated kinase
  • protein tyrosine kinase ERK2
Background InformationErk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).
References
Product Information
FormatPurified
Control
  • RIPA cell lysate from NGF stimulated PC-12 cells
PresentationCultured supernantant in 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationDetect phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) using this Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39 validated for use in Mplex & WB.
Key Applications
  • Multiplexing
  • Western Blotting
Application NotesWestern Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte® Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex® microspheres. (Figure B).
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.
CloneAw39
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman and rat. Predicted to cross-react with many species based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Gene Symbol
  • MAPK1
  • p38
  • ERK
  • P42MAPK
  • ERK2
  • p40
  • PRKM1
  • p41mapk
  • PRKM2
  • MAPK2
  • p42-MAPK
  • p41
  • ERK-2
  • ERT1
Modifications
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular WeightMAPK1 (~44 kDa) and MAPK2 (~42 kDa).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-797 04053252670633

Documentation

Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39, rabbit monoclonal MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - 2441925 2441925
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - 3149841 3149841
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - 3415971 3415971
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - 3589755 3589755
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - 4079018 4079018
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - DAM1502443 DAM1502443
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2608736 2608736
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2650796 2650796
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2714510 2714510
Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2739248 2739248
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