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07-833 Anti-phospho-Gab1 (Tyr627) Antibody

This Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).

Anti-phospho-Gab1 (Tyr627) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: GAB1 UniProt Number: Q13480
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07-833
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-833
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Gab1 (Tyr627) Antibody
Alternate Names
  • GAB1
  • GRB2-associated binding protein 1
References
Product Information
FormatAffinity Purified
Presentation100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).
Key Applications
  • Western Blotting
Application NotesImmunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Gab1 (Tyr627) in RIPA lysate of PDGF treated NIH3T3 cells.
Biological Information
ImmunogenPeptide corresponding to amino acid region encompassing the human and mouse phospho-Gab1 (Tyr627)
HostRabbit
SpecificityGab1 phosphorylated on Tyrosine residue 627.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NotePredicted to cross-react with human based on 100% sequence homology, but have not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. The encoded protein is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • GAB1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13480 # Probably involved in EGF and insulin receptor signaling.
SIZE: 694 amino acids; 76616 Da
SUBUNIT: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2.
PTM: Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
SIMILARITY: SwissProt: Q13480 ## Belongs to the GAB family. & Contains 1 PH domain.
Molecular Weight115 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-833 04053252469459

Documentation

Anti-phospho-Gab1 (Tyr627) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Gab1 (Tyr627) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-Gab1 (Tyr627) (Affinity purified Rabbit polyclonal IgG) 3115880
Anti-phospho-Gab1 (Tyr627) (Affinity purified Rabbit polyclonal IgG) - DAM1465948 DAM1465948
Anti-phospho-Gab1 (Tyr627) - 1988572 1988572
Anti-phospho-Gab1 (Tyr627) - DAM1413923 DAM1413923
anti-phospho-Gab1 (Tyr627) - 0604027241 0604027241
anti-phospho-Gab1 (Tyr627) - 0604027242 0604027242
anti-phospho-Gab1 (Tyr627) - 0610042766 0610042766
anti-phospho-Gab1 (Tyr627) - 0612047427 0612047427
anti-phospho-Gab1 (Tyr627) - JBC1378200 JBC1378200

References

Reference overviewPub Med ID
Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progressio
Bourguignon, Lilly Y W, et al
J Biol Chem, 278:29420-34 (2003)  2003

12748184 12748184
Roles of cell-cell adhesion-dependent tyrosine phosphorylation of Gab-1
Shinohara, M, et al
J Biol Chem, 276:18941-6 (2001)  2001

11262408 11262408
Induction of epithelial tubules by growth factor HGF depends on the STAT pathway
Boccaccio, C, et al
Nature, 391:285-8 (1998)  1998

9440692 9440692
A Grb2-associated docking protein in EGF- and insulin-receptor signalling
Holgado-Madruga, M, et al
Nature, 379:560-4 (1996)  1996

8596638 8596638