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05-740-AF488 Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 is an antibody against phospho-ATM (Ser1981) for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: ATM, AT UniProt Number: Q13315
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05-740-AF488
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-740-AF488
DescriptionAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488
Alternate Names
  • Serine-protein kinase ATM, Ser1981-phosphorylated
  • Ataxia telangiectasia mutated, Ser1981-phosphorylated
  • A-T mutated, Ser1981-phosphorylated
  • ATM, Ser1981-phosphorylated
Background InformationSerine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 is an antibody against phospho-ATM (Ser1981) for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antibody (Cat. Nos. 05-740, MAB3806 & MAB3806-C) is shown to be suitable also for ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting applications. PE-conjugate (Cat. No. FCMAB110P) is also available and tested for Flow cytometry application.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence is 11/12 identical to the corresponding mouse and rat sequence.
EpitopepSer1981.
Clone10H11.E12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityReacts with ATM phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATM
  • AT
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
UniProt Number
Molecular WeightThe unconjugated antibody (Cat. No. 05-430) is shown to recognize the ~370 kDa phosphoATM target band and a >400 kDa non-specific band.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected UV-induced ATM Ser1981 phosphorylation in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-740-AF488 04055977350586

Documentation

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 Certificates of Analysis

TitleLot Number
Anti-phospho-ATM (Ser1981), -Q2683363 Q2683363
Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 - 3308163 3308163
Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 - 3757087 3757087
Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 - 4203377 4203377
Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 -2890748 2890748
Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 Monoclonal Antibody 3123163